Anticorps Polyclonal de lapin anti-NMDAR2A/GRIN2A

NMDAR2A/GRIN2A Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

25

Avis (1)

N° de cat : 19953-1-AP

Synonymes

GluN2A, GRIN2A, hNR2A, NMDAR2A, NMDAR2A/GRIN2A, NR2A

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Galerie de données de validation

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  WB analysis of mouse brain using 19953-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 19953-1-AP (NMDAR2A/GRIN2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 19953-1-AP

  IP result of anti-NMDAR2A/GRIN2A(IP:19953-1-AP, 4ug; Detection:19953-1-AP 1:600) with mouse brain tissue lysate 1680 ug.

• 

  IHC staining of mouse brain using 19953-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19953-1-AP (NMDAR2A/GRIN2A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 19953-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19953-1-AP (NMDAR2A/GRIN2A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 19953-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19953-1-AP (NMDAR2A/GRIN2A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 19953-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19953-1-AP (NMDAR2A/GRIN2A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 19953-1-AP

  Immunofluorescent analysis of Hela cells, using NMDAR2A/GRIN2A antibody 19953-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  FC experiment of SH-SY5Y using 19953-1-AP

  1X10^6 SH-SY5Y cells were stained with 0.2ug NMDAR2A/GRIN2A antibody (19953-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris
• Résultats positifs en IP: tissu cérébral de souris,
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa
• Résultats positifs en cytométrie: cellules SH-SY5Y

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF): IF : 1:10-1:100
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 25 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

19953-1-AP cible NMDAR2A/GRIN2A dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: glutamate receptor, ionotropic, N-methyl D-aspartate 2A
• Masse moléculaire calculée: 165 kDa
• Poids moléculaire observé: 150-160 kDa
• Numéro d’acquisition GenBank: NM_000833
• Symbole du gène: GRIN2A
• Identification du gène (NCBI): 2903
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GRIN2A, also named as NMDAR2A and NR2A, belongs to the glutamate-gated ion channel (TC 1.A.10) family. GRIN2A is NMDA receptor subtype of glutamate-gated ion channels possesses high calcium permeability and voltage-dependent sensitivity to magnesium. Activation of GRIN2A requires binding of agonist to both types of subunits. The antibody recognize the C-term of GRIN2A.

Publications

Species	Application	Title
mouse	WB	Sci Adv; Loss of schizophrenia-related miR-501-3p in mice impairs sociability and memory by enhancing mGluR5-mediated glutamatergic transmission; Authors - Wenquan Liang
mouse	WB	Sci Adv; RNF220 is an E3 ubiquitin ligase for AMPA receptors to regulate synaptic transmission; Authors - Pengcheng Ma
mouse	WB	Mol Psychiatry; A recurrent SHANK1 mutation implicated in autism spectrum disorder causes autistic-like core behaviors in mice via downregulation of mGluR1-IP3R1-calcium signaling.; Authors - Yue Qin
human	WB	J Clin Invest; TMEM25 modulates neuronal excitability and NMDA receptor subunit NR2B degradation.; Authors - Haiqing Zhang
human	WB	EBioMedicine; Inhibition of Nwd1 activity attenuates neuronal hyperexcitability and GluN2B phosphorylation in the hippocampus.; Authors - Qin Yang
rat	WB	Aging (Albany NY); Enriched environment remedies cognitive dysfunctions and synaptic plasticity through NMDAR-Ca2+-Activin A circuit in chronic cerebral hypoperfusion rats.; Authors - Xin Zhang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Lisa (Verified Customer) (10-04-2021)

The antibody works quite good on hiPSCs-derived neurons. The signal is strong. The obtained bands are not very specific but the signal itself it is, therefore is easy to analyze the data.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: hiPSCs-derived neurons

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