Anticorps Polyclonal de lapin anti-HCN2

HCN2 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 55245-1-AP

Synonymes

HAC 1, HCN2

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Galerie de données de validation

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  WB analysis of mouse brain using 55245-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55245-1-AP (HCN2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 55245-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 55245-1-AP (HCN2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 55245-1-AP

  IP Result of anti-HCN2 (IP:55245-1-AP, 4ug; Detection:55245-1-AP 1:800) with mouse brain tissue lysate 4000ug.

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  IHC staining of human heart using 55245-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 55245-1-AP (HCN2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human brain using 55245-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 55245-1-AP (HCN2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 55245-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 55245-1-AP (HCN2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 55245-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 55245-1-AP (HCN2 antibody) at dilution of 1:50 (under 10x lens).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules Jurkat
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu cardiaque humain, tissu cérébral humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

55245-1-AP cible HCN2 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: hyperpolarization activated cyclic nucleotide-gated potassium channel 2
• Masse moléculaire calculée: 97 kDa
• Poids moléculaire observé: 97-110 kDa
• Numéro d’acquisition GenBank: NM_001194
• Symbole du gène: HCN2
• Identification du gène (NCBI): 610
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HCN2, also named as BCNG2, belongs to the potassium channel HCN family. It hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. Contributes to the native pacemaker currents in heart (If) and in neurons (Ih). HCN2 produces a large instantaneous current. It is activated by cAMP. HCN2 is modulated by intracellular chloride ions and pH; acidic pH shifts the activation to more negative voltages. This antibody is specific to HCN2.

Publications

Species	Application	Title
human	WB,IF	Nucleic Acids Res; Non-CpG methylation by DNMT3B facilitates REST binding and gene silencing in developing mouse hearts.; Authors - Donghong Zhang
rat	WB	Mol Pain; HCN2 contributes to oxaliplatin-induced neuropathic pain through activation of the CaMKII/CREB cascade in spinal neurons.; Authors - Xiaoyu Liu
rat	WB,IHC	Brain Res Bull; HCN2 contributes to oxaliplatin-induced neuropathic pain by inducing spinal long-term potentiation via activation of NMDA receptor-mediated CaMKII signaling.; Authors - Xiaoyu Liu
mouse	WB	Exp Ther Med; Effect of mHCN2 gene modification on chronotropic relevant receptors in BMSCs co-cultured with atrial myocytes.; Authors - Chandong Ding
mouse	WB	Cell Rep; Optogenetic Activation of Striatopallidal Neurons Reveals Altered HCN Gating in DYT1 Dystonia.; Authors - Giuseppe Sciamanna
mouse	WB	Neurosci Bull; Thalamocortical Circuit Controls Neuropathic Pain via Up-regulation of HCN2 in the Ventral Posterolateral Thalamus; Authors - Yi Yan ; KD Validated