Tested Applications
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-84316-7 targets HNF4A in IF/ICC applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag24647 Product name: Recombinant human HNF4A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-350 aa of BC137539 Sequence: MRLSKTLVDMDMADYSAALDPAYTTLEFENVQVLTMGNDTSPSEGTNLNAPNSLGVSALCAICGDRATGKHYGASSCDGCKGFFRRSVRKNHMYSCRFSRQCVVDKDKRNQCRYCRLKKCFRAGMKKEAVQNERDRISTRRSSYEDSSLPSINALLQAEVLSRQITSPVSGINGDIRAKKIASIADVCESMKEQLLVLVEWAKYIPAFCELPLDDQVALLRAHAGEHLLLGATKRSMVFKDVLLLGNDYIVPRHCPELAEMSRVSIRILDELVLPFQELQIDDNEYAYLKAIIFFDPDAKGLSDPGKIKRLRSQVQVSLEDYINDRQYDSRGRFGELLLLLPTLQSITWQ Predict reactive species |
| Full Name | hepatocyte nuclear factor 4, alpha |
| Calculated Molecular Weight | 53 kDa |
| GenBank Accession Number | BC137539 |
| Gene Symbol | HNF4A |
| Gene ID (NCBI) | 3172 |
| RRID | AB_3747530 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Excitation Laser | Blue laser (488 nm) |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P41235 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
HNF4A (Hepatocyte nuclear factor 4 α) is highly conserved between humans and mice. Using conditional knockouts, epithelial HNF4A is indispensable for protecting against acute chemical-induced colitis and spontaneous colonic inflammation in aged animals (PMID:35792863). Moreover, reduced expression of HNF4A has been reported in liver fibrosis and viral vector-mediated HNF4A restoration has previously been demonstrated to reverse features of chronic liver injury. Therefore, HNF4A has been suggested as a therapeutic target for the treatment of liver fibrosis (PMID:34453962).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 HNF4A antibody CL488-84316-7 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
What customers say
The single review for CL488-84316-7 is a 5-star rating for immunofluorescence on human liver tissue at a 1:100 dilution. The reviewer reported strong, specific nuclear staining in hepatocytes with minimal background and a clean signal-to-noise ratio, making it easy to distinguish positive nuclei from surrounding tissue. Overall, the antibody was recommended for liver IF applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Jyoti (Verified Customer) (07-17-2026) | Worked really well for IF on human liver tissue at 1:100 dilution. Strong, specific nuclear staining in hepatocytes with minimal background. Clean signal-to-noise ratio, easy to distinguish positive nuclei from surrounding tissue. Would recommend for liver IF applications.
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