Anticorps Polyclonal de lapin anti-HNRNPR

• Phare
• Validé par KD/KO

HNRNPR Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 15018-1-AP

Synonymes

hnRNP R, HNRNPR, HNRPR

---

Galerie de données de validation

• 

  WB analysis of HepG2 using 15018-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15018-1-AP (HNRNPR antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15018-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15018-1-AP (HNRNPR antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human brain using 15018-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15018-1-AP (HNRNPR antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 15018-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15018-1-AP (HNRNPR antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HepG2 using 15018-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 15018-1-AP (HNRNPR antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Applications testées

• Résultats positifs en WB: cellules HepG2, cellules HeLa
• Résultats positifs en IHC: tissu cérébral humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 2 publications below

Informations sur le produit

15018-1-AP cible HNRNPR dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HNRNPR Protéine recombinante Ag7028
• Nom complet: heterogeneous nuclear ribonucleoprotein R
• Masse moléculaire calculée: 71 kDa
• Poids moléculaire observé: 65-82 kDa
• Numéro d’acquisition GenBank: BC001449
• Symbole du gène: HNRNPR
• Identification du gène (NCBI): 10236
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HNRNPR belongs to Heterogeneous nuclear ribonucleoproteins (hnRNPs), which are among the most abundant proteins in the eukaryotic nucleus and they play important roles in processing of precursor mRNA. hnRNP proteins have a modular structure consisting of one or more RNA binding domains and at least another auxiliary domain, which likely mediates protein-protein interactions but may also contribute to nucleic acid binding. The calcualted molecular weight of HNRNPR is 71 kDa, but the migration behavior of the 82 kDa translation product was indistinguishable from that of HNRNPR. (PMID: 9421497)

Publications

Species	Application	Title
human	WB	J Immunol; HNRNPR Regulates the Expression of Classical and Nonclassical MHC Class I Proteins.; Authors - Adi Reches ; KD Validated
mouse	WB	Neurosci Lett; hnRNPM deficiency leads to cognitive deficits via disrupting synaptic plasticity.; Authors - Akinsola Raphael Akinyemi
human	IHC,WB	Front Oncol; The hnRNPK/A1/R/U Complex Regulates Gene Transcription and Translation and is a Favorable Prognostic Biomarker for Human Colorectal Adenocarcinoma.; Authors - Yixin Li
	WB	iScience; Increased LDL receptor by SREBP2 or SREBP2-induced lncRNA LDLR-AS promotes triglyceride accumulation in fish; Authors - Xiufei Cao ; KD Validated
human	IHC	J Transl Med; High expression of HNRNPR in ESCA combined with 18F-FDG PET/CT metabolic parameters are novel biomarkers for preoperative diagnosis of ESCA; Authors - Xiao-Yu Liu
human	WB	Eur J Pharm Sci; Novel facile thermosensitive hydrogel as sustained and controllable gene release vehicle for breast cancer treatment.; Authors - Dan Zhao