Anticorps Polyclonal de lapin anti-HYAL2

• Phare
• Validé par KD/KO

HYAL2 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(1)

N° de cat : 51148-1-AP

Synonymes

Hyal 2, HYAL2, Hyaluronidase 2, hyaluronoglucosaminidase 2, LuCa 2, LUCA2, Lung carcinoma protein 2

---

Galerie de données de validation

• 

  WB analysis of mouse heart using 51148-1-AP

  mouse heart tissue and mouse liver tissue were subjected to SDS PAGE followed by western blot with 51148-1-AP (HYAL2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 51148-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 51148-1-AP (HYAL2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human lung using 51148-1-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 51148-1-AP (HYAL2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 51148-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 51148-1-AP (HYAL2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 51148-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 51148-1-AP (HYAL2 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 51148-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 51148-1-AP (HYAL2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

---

Applications testées

• Résultats positifs en WB: tissu cardiaque de souris, tissu cardiaque humain, tissu hépatique de souris, tissu pulmonaire humain
• Résultats positifs en IHC: tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below

Informations sur le produit

51148-1-AP cible HYAL2 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HYAL2 Protéine recombinante Ag0582
• Nom complet: hyaluronoglucosaminidase 2
• Masse moléculaire calculée: 54 kDa
• Poids moléculaire observé: 60 kDa
• Numéro d’acquisition GenBank: BC000692
• Symbole du gène: HYAL2
• Identification du gène (NCBI): 8692
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Hyaluronic acid (HA) is a glycosaminoglycan that is believed to have numerous important biologic functions, including modulation of cell proliferation, migration, and differentiation, as well as the regulation of extracellular water and protein homeostasis. It is also an integral structural component of cartilage and other tissues and acts as a lubricant in joints. Hyaluronidases are a family of enzymes that catalyse the degradation of HA. In humans, there are five functional hyaluronidases: HYAL1, HYAL2, HYAL3, HYAL4 and HYAL5 (also known as SPAM1 or PH-20); plus a pseudogene, HYAL6 (also known as HYALP1). HYAL2 is present in many tissues except the adult brain (PMID: 11731268). HYAL2 can be exposed to cell surfaces that bind to the plasma membrane via GPI anchors (PMID: 11731268).

Publications

Species	Application	Title
human	WB,IHC	Dev Cell; High-Molecular-Weight Hyaluronan Is a Hippo Pathway Ligand Directing Cell Density-Dependent Growth Inhibition via PAR1b.; Authors - Takuya Ooki ; KD Validated

{{ptg:RelatedPrimaryAntibodies}}