Tested Applications
| Positive WB detected in | HEK-293 cells, A-673 cells, HeLa cells, NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
28348-1-AP targets Integrin alpha-9 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag28815 Product name: Recombinant human Integrin alpha-9 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 462-613 aa of BC030198 Sequence: VITVDVSIFLPGSINITAPQCHDGQQPVNCLNVTTCFSFHGKHVPEEIGLNYVLMADVAKKEKGQMPRVYFVLLGETMGQVTEKLQLTYMEETCRHYVAHVKRRVQDVISPIVFEAAYSLSEHVTGEEERELPPLTPVLRWKKGQKIAQKNQ Predict reactive species |
| Full Name | integrin, alpha 9 |
| Calculated Molecular Weight | 114 kDa |
| Observed Molecular Weight | 140-150 kDa |
| GenBank Accession Number | BC030198 |
| Gene Symbol | Integrin alpha 9 |
| Gene ID (NCBI) | 3680 |
| RRID | AB_3669655 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13797 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). Integrin alpha 9 contains a large N-terminal extracellular domain with 7 conserved repeats of putative metal-binding domains, a transmembrane segment, and a short C-terminal cytoplasmic tail (PMID: 8245132). Integrin alpha 9 interacts with integrin beta 1, generating α9β1 heterodimer, which is expressed in many cell types, including epithelial cells, neutrophils, hepatocytes, muscle, and endothelial cells (PMID: 21975548). It binds to a diversity of ligands in the extracellular matrix, like the a-disintegrin and metalloprotease (ADAM) family, THBS1, EMILIN1, VEGF, tenascin-C, osteopontin, fibronectin, and VCAM1 (PMID: 21975548; 33790909). Integrin α9β1 has been implicated in diverse biological functions, including angiogenesis, lymphangiogenesis, lymphatic valve morphogenesis, granulopoiesis and tumorigenesis (PMID: 20179422).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Integrin alpha-9 antibody 28348-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Integrin alpha-9 Polyclonal antibody (Cat# 28348-1-AP) has 1 total citation. It appears in Materials Today Bio (IF 11), a 2026 study by Zongbo Dai et al. The research investigates how a decellularized extracellular matrix/GelMA composite hydrogel derived from hepatic cavernous hemangioma promotes angiogenesis via the ITGA9-FAK-ERK1/2 signaling axis. The antibody was used for Western blot and immunohistochemistry in human and mouse samples, with RNAi validation. The associated research fields include angiogenesis, tissue engineering, and biomaterials.
| Species | Application | Title |
|---|---|---|
Mater Today Bio Hepatic cavernous hemangioma decellularized extracellular matrix/GelMA composite hydrogel promotes angiogenesis via the ITGA9-FAK-ERK1/2 axis.
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