Anticorps Polyclonal de lapin anti-KDM3A,JMJD1A

• Phare
• Validé par KD/KO

KDM3A,JMJD1A Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IP, IHC, IF, CoIP, ChIP, ELISA

Conjugaison

Non conjugué

Publications

38

N° de cat : 12835-1-AP

Synonymes

JHDM2A, JHMD2A, JMJD1, JMJD1A, KDM3A, KDM3A,JMJD1A, KIAA0742, lysine (K) demethylase 3A, Lysine specific demethylase 3A, TSGA

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Galerie de données de validation

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  WB analysis of HeLa using 12835-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12835-1-AP (KDM3A,JMJD1A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 12835-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12835-1-AP (KDM3A,JMJD1A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 12835-1-AP

  IP Result of anti-KDM3A,JMJD1A (IP:12835-1-AP, 5ug; Detection:12835-1-AP 1:1000) with HeLa cells lysate 2500ug.

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  IHC staining of human ovary tumor using 12835-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 12835-1-AP (KDM3A,JMJD1A antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary using 12835-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 12835-1-AP (KDM3A,JMJD1A antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary using 12835-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 12835-1-AP (KDM3A,JMJD1A antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 12835-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 12835-1-AP (KDM3A,JMJD1A antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 12835-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 12835-1-AP (KDM3A,JMJD1A antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 12835-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 12835-1-AP (KDM3A,JMJD1A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 12835-1-AP

  Immunofluorescent analysis of HepG2 cells, using KDM3A antibody 12835-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules MCF-7
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de tumeur ovarienne humain, tissu ovarien humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 15 publications below
• WB: See 32 publications below
• IHC: See 10 publications below
• IF: See 4 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below
• ChIP: See 6 publications below

Informations sur le produit

12835-1-AP cible KDM3A,JMJD1A dans les applications de WB, IP, IHC, IF, CoIP, ChIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: KDM3A,JMJD1A Protéine recombinante Ag3581
• Nom complet: lysine (K)-specific demethylase 3A
• Masse moléculaire calculée: 150 kDa
• Poids moléculaire observé: 150 kDa
• Numéro d’acquisition GenBank: BC038297
• Symbole du gène: KDM3A
• Identification du gène (NCBI): 55818
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

KDM3A, also name as JMJD1A, JHDM2A, JMJD1, JMJD1A, KIAA0742 and TSGA, belongs to the JHDM2 histone demethylase family. It is a histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. It is involved in spermatogenesis by regulating expression of target genes such as PRM1 and TMP1 which are required for packaging and condensation of sperm chromatin. It is involved in obesity resistance through regulation of metabolic genes such as PPARA and UCP1. (PubMed:16603237), JMJD1A is a useful biomarker for hypoxic tumor cells and a prognostic marker that could be a promising therapeutic target against CRC. (PMID:20823141) This antibody recognize the N-term of KDM3A(JMJD1A).

Publications

Species	Application	Title
human	WB,IHC,IF	Nat Commun; Normal stroma suppresses cancer cell proliferation via mechanosensitive regulation of JMJD1a-mediated transcription.; Authors - Riina Kaukonen ; KD Validated
human	WB	Nat Commun; Delaying histone deacetylase response to injury accelerates conversion into repair Schwann cells and nerve regeneration.; Authors - Valérie Brügger
human	WB	Biomaterials; Lysine demethylase KDM3A regulates nanophotonic hyperthermia resistance generated by 2D silicene in breast cancer.; Authors - Chao He
mouse	WB	EMBO J; Coordinated removal of repressive epigenetic modifications during induced reversal of cell identity.; Authors - Khoa A Tran
human	WB	Proc Natl Acad Sci U S A; Histone demethylase JMJD1A promotes alternative splicing of AR variant 7 (AR-V7) in prostate cancer cells.; Authors - Lingling Fan ; KD Validated
mouse	WB,IF	J Cell Biol; KDM3A coordinates actin dynamics with intraflagellar transport to regulate cilia stability.; Authors - Patricia L Yeyati ; KO Validated

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