Anticorps Recombinant de lapin anti-LAG-3/CD223

LAG-3/CD223 Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC

Conjugaison

Non conjugué

CloneNo.

243086D4

N° de cat : 98435-2-RR

Synonymes

CD223, LAG3, FDC, LAG 3, LAG-3

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Galerie de données de validation

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  FC experiment of human PBMCs using 98435-2-RR

  1x10^6 untreated or anti-CD3/CD28 and IL-2 treated human PBMCs were surface stained with 0.25 ug Anti-Human LAG-3/CD223 Rabbit RecAb (98435-2-RR, Clone: 243086D4) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD8 (UCHT4) Mouse IgG2a Recombinant Antibody (CL647-65559, Clone: UCHT4). Cells were not fixed.

• 

  FC experiment of human PBMCs using 98435-2-RR

  1x10^6 anti-CD3/CD28 and IL-2 treated human PBMCs were surface stained with 0.25 ug Anti-Human LAG3 Rabbit RecAb (98435-2-RR, Clone: 243086D4) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated human PBMCs were surface stained with 0.25 ug Anti-Human LAG3 Rabbit RecAb (98435-2-RR, Clone: 243086D4) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (black, dashed). Cells were not fixed.

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  Affinity and Kinetic Characterization of 98435-2-RR

  Biolayer interferometry (BLl) kinetic assays of 98435-2-RR against Human LAG-3/CD223 were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en cytométrie: Anti-CD3/CD28 and IL-2 treated human PBMCs

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

98435-2-RR cible LAG-3/CD223 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: LAG-3/CD223 Protéine recombinante Eg31550
• Nom complet: lymphocyte-activation gene 3
• Masse moléculaire calculée: 57 kDa
• Numéro d’acquisition GenBank: NM_002286
• Symbole du gène: LAG-3
• Identification du gène (NCBI): 3902
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2 - 8°C. Stable for one year after shipment.

Informations générales

LAG-3, also known as CD223, is an immune checkpoint molecule that regulates both T-cell activation and homeostasis. LAG-3 is expressed on activated T cells, NK cells, regulatory T cells, and plasmacytoid dendritic cells. It is a CD4-related molecule that binds MHC class II. LAG-3 plays an important role in modulating T cell expansion and function, and blockade of LAG-3 with monoclonal antibodies can augment T cell function. (PMID: 15634887; 21086108; 28783703)

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