Anticorps Polyclonal de lapin anti-LCK

• Phare
• Validé par KD/KO

LCK Polyclonal Antibody for IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 12477-1-AP

Synonymes

LCK, LSK, p56 LCK, p56lck, pp58lck, Protein YT16, Proto oncogene Lck, Tyrosine protein kinase Lck, YT16

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Galerie de données de validation

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  WB analysis of Jurkat using 12477-1-AP

  WB result of LCK antibody (12477-1-AP, 1:2000) with si-control and si-LCK transfected Jurkat cells.

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  WB analysis of Jurkat using 12477-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12477-1-AP (LCK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 12477-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12477-1-AP (LCK antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 12477-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12477-1-AP (LCK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 12477-1-AP

  IP Result of anti-LCK (IP:12477-1-AP, 4ug; Detection:12477-1-AP 1:500) with Jurkat cells lysate 4000ug.

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules Raji, cellules Ramos
• Résultats positifs en IP: cellules Jurkat

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below

Informations sur le produit

12477-1-AP cible LCK dans les applications de WB, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: LCK Protéine recombinante Ag3176
• Nom complet: lymphocyte-specific protein tyrosine kinase
• Masse moléculaire calculée: 539 aa, 56 kDa
• Poids moléculaire observé: 50-60 kDa
• Numéro d’acquisition GenBank: BC013200
• Symbole du gène: LCK
• Identification du gène (NCBI): 3932
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

LCK(Lymphocyte cell-specific protein-tyrosine kinase) is also named as LSK,p56-LCK and a nonreceptor protein-tyrosine kinase of the SRConcogene family that is involved in transduction of T-cell receptor-mediated activation.It is implicated in the control of lymphocyte growth by virtue of its overexpression in some lymphoid malignancies and its transforming activity in heterologous systems(PMID:2784463).It has 3 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human	WB	J Ovarian Res; Pretreatment with LCK inhibitors chemosensitizes cisplatin-resistant endometrioid ovarian tumors.; Authors - Katie K Crean-Tate
mouse	WB	Front Pharmacol; "Drying effect" of fructus aurantii components and the mechanism of action based on network pharmacology and in vitro pharmacodynamic validation; Authors - Jing Zhu
human	WB	J Ovarian Res; LCK facilitates DNA damage repair by stabilizing RAD51 and BRCA1 in the nucleus of chemoresistant ovarian cancer; Authors - Goutam Dey
mouse	WB	Nat Commun; Cell volume controlled by LRRC8A-formed volume-regulated anion channels fine-tunes T cell activation and function; Authors - Yuman Wang