Anticorps Polyclonal de lapin anti-Lipocalin-2/NGAL

• Phare
• Validé par KD/KO

Lipocalin-2/NGAL Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(4)

N° de cat : 30700-1-AP

Synonymes

LCN2, lipocalin 2, NGAL, 24p3, 25 kDa alpha-2-microglobulin-related subunit of MMP-9

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Galerie de données de validation

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  WB analysis using 30700-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30700-1-AP (Lipocalin-2/NGAL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HT-29 using 30700-1-AP

  WB result of NGAL antibody (30700-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NGAL transfected HT-29 cells.

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  IHC staining of human colon cancer using 30700-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 30700-1-AP (Lipocalin-2/NGAL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 30700-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 30700-1-AP (LCN2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of Human bowens disease using 30700-1-AP

  Immunohistochemical analysis of paraffin-embedded human brown disease slide using 30700-1-AP (NGAL antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of RAW 264.7 using 30700-1-AP

  Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using Lipocalin-2/NGAL antibody (30700-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HT-29,
• Résultats positifs en IHC: tissu de cancer du côlon humain, Human bowens disease; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules RAW 264.7,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

30700-1-AP cible Lipocalin-2/NGAL dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Lipocalin-2/NGAL Protéine recombinante Ag33369
• Nom complet: lipocalin 2
• Poids moléculaire observé: 22-25 kDa
• Symbole du gène: NGAL/LCN2
• Identification du gène (NCBI): 3934
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Lipocalin-2 (LCN-2) is a adipocytokine also referred to as neutrophil gelatinase-associated lipocalin (NGAL). Lipocalin-2 is a circulatory protein responsible for the transportation of small and hydrophobic molecules to target organs. Lipocalin-2 is used as a biomarker for acute and chronic renal injury. It is present in a large variety of cells including neutrophil, hepatocytes, lung, bone marrow, adipose tissue, macrophages, thymus, non-neoplastic breast duct, prostate, and renal cells. Different functions have been associated with Lipocalin-2, including antibacterial, anti-inflammatory, and protection against cell and tissue stress (PMID:34463264).

Publications

Species	Application	Title
mouse	IHC	Redox Biol; Protective role of vitamin D receptor against mitochondrial calcium overload from PM2.5-Induced injury in renal tubular cells; Authors - Mengqiu Lu
mouse	IF	Front Bioeng Biotechnol; Ultra-small curcumin-ruthenium coordination polymer nanodots prevent renal ischemia-reperfusion injury and the progression to chronic kidney disease; Authors - Xian Liu
mouse	WB	Adv Sci (Weinh); Adaptive Antioxidant Nanomedicines Inhibit Ferroptosis in Renal Tubular Epithelial Cells to Alleviate Diabetic Kidney Disease; Authors - Zerun Liu
mouse	IHC	Front Mol Biosci; S100A8/A9 high-expression macrophages mediate renal tubular epithelial cell damage in acute kidney injury following acute type A aortic dissection surgery; Authors - Xiujuan Cai

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