Anticorps Polyclonal de lapin anti-EDG2

EDG2 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 20442-1-AP

Synonymes

edg 2, EDG2, Gpcr26, GPR26, LPA 1, LPA receptor 1, LPA1, LPAR1, Mrec1.3, rec.1.3, vzg 1, VZG1

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Galerie de données de validation

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  WB analysis of Jurkat using 20442-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 20442-1-AP (EDG2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of multi-cells/tissue using 20442-1-AP

  Multi-cells/tissue were subjected to SDS PAGE followed by western blot with 20442-1-AP (EDG2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 20442-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20442-1-AP (EDG2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 20442-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 20442-1-AP (EDG2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 20442-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20442-1-AP (EDG2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 20442-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20442-1-AP (EDG2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 20442-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20442-1-AP (EDG2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 20442-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 20442-1-AP (LPAR1,EDG2 Antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 20442-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 20442-1-AP (LPAR1,EDG2 Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules A2780, cellules A375, cellules A549, cellules HeLa, cellules NIH3T3, multi-cellules/tissus, tissu cérébral de souris
• Résultats positifs en IHC: tissu cérébral humain, tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:300-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

20442-1-AP cible EDG2 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EDG2 Protéine recombinante Ag14253
• Nom complet: lysophosphatidic acid receptor 1
• Masse moléculaire calculée: 364 aa, 41 kDa
• Poids moléculaire observé: 41-50 kDa
• Numéro d’acquisition GenBank: BC030615
• Symbole du gène: LPAR1
• Identification du gène (NCBI): 1902
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

EDG2 (also known as LPA1) is a G-protein coupled receptor for lysophosphatidic acid (LPA), a potent motility inducing factor, which is a major component of serum (PMID: 19415462). EDG2 is widely expressed in normal tissue during growth and development. In the context of cancer, several studies have suggested that EDG2 expression in tumors is often similar to that shown in normal tissue (PMID: 17496233).

Publications

Species	Application	Title
human	WB	Mediators Inflamm; AM966, an Antagonist of Lysophosphatidic Acid Receptor 1, Increases Lung Microvascular Endothelial Permeability through Activation of Rho Signaling Pathway and Phosphorylation of VE-Cadherin.; Authors - Junting Cai

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