Anticorps Monoclonal anti-MHC Class II (I-A/I-E)

MHC Class II (I-A/I-E) Monoclonal Antibody for FC

Hôte / Isotype

Rat / IgG2b, kappa

Réactivité testée

souris

Applications

FC

Conjugaison

APC Fluorescent Dye

Publications(2)

CloneNo.

M5/114.15.2

N° de cat : APC-65122

Synonymes

H2Aa, Aalpha, H 2Aa, H2-Aa, Ia 1

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Galerie de données de validation

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  FC experiment of BALB/c mouse splenocytes using APC-65122

  1x10^6 BALB/c mouse splenocytes were surface stained with 0.125 ug APC Anti-Mouse MHC Class II (I-A/I-E) (APC-65122, Clone: M5/114.15.2) or 0.125 ug APC Rat IgG2b Isotype Control (LTF-2) (APC-65211, Clone: LTF-2). Cells were co-stained with PE Anti-Mouse CD3 (17A2) (PE-65077, Clone: 17A2). Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: splénocytes de souris BALB/c

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Applications publiées

• FC: See 2 publications below

Informations sur le produit

APC-65122 cible MHC Class II (I-A/I-E) dans les applications de FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Réactivité citée: souris
• Hôte / Isotype: Rat / IgG2b, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Cellules spléniques activées de souris C57BL/6
• Nom complet: histocompatibility 2, class II antigen A, alpha
• Numéro d’acquisition GenBank: BC031711
• Symbole du gène: MHC Class II
• Identification du gène (NCBI): 14960
• Conjugaison: APC Fluorescent Dye
• Excitation/Emission maxima wavelengths: 650 nm / 660 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

Major histocompatibility complex (MHC) class II molecules are heterodimeric transmembrane glycoproteins that are expressed on the surface of antigen-presenting cells such as dendritic cells, macrophages, and B cells. The M5/114.15.2 antibody detects polymorphic determinants present on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice carrying the H-2b, H-2d, H-2q, H-2p, H-2r and H-2u haplotypes but not from mice carrying the H-2s or H-2f haplotypes (PMID: 6170707). This antibody recognizes both I-A and I-E subregion-encoded Ia glycoproteins (I-Ab, I-Ad, I-Aq, I-Ed, I-Ek, not I-Af, I-Ak, or I-As). M5/114 mAb is reported to inhibit I-A subregion-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not the H-2f, H-2k, H-2s haplotypes (PMID: 6173436).

Publications

Species	Application	Title
mouse	FC	BMC Ophthalmol; Do the monocyte-derived dendritic cells exert a pivotal role in the early onset of experimental autoimmune uveitis; Authors - Bo Yuan
mouse	FC	Nutrients; The Potential Role of Moringa oleifera Lam. Leaf Proteins in Moringa Allergy by Functionally Activating Murine Bone Marrow-Derived Dendritic Cells and Inducing Their Differentiation toward a Th2-Polarizing Phenotype; Authors - Chuyu Xi

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