Anticorps Polyclonal de lapin anti-MOGAT2

MOGAT2 Polyclonal Antibody for IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 19514-1-AP

Synonymes

DC5, DGAT2L5, hDC5, hMGAT2, MGAT2, MOGAT2

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Galerie de données de validation

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  WB analysis using 19514-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19514-1-AP (MOGAT2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 19514-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 19514-1-AP (MOGAT2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 19514-1-AP

  IP Result of anti-MOGAT2 (IP:19514-1-AP, 4ug; Detection:19514-1-AP 1:300) with HEK-293 cells lysate 4800ug.

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Applications testées

• Résultats positifs en WB: tissu d'intestin grêle de souris, cellules HEK-293, intestin grêle de rat
• Résultats positifs en IP: cellules HEK-293

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 7 publications below

Informations sur le produit

19514-1-AP cible MOGAT2 dans les applications de WB, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: monoacylglycerol O-acyltransferase 2
• Masse moléculaire calculée: 38 kDa
• Poids moléculaire observé: 36-38 kDa
• Numéro d’acquisition GenBank: NM_025098
• Symbole du gène: MOGAT2
• Identification du gène (NCBI): 80168
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MOGAT2, also named as DC5 and DGAT2L5, belongs to the diacylglycerol acyltransferase family. MOGAT2 catalyze the reaction: Acyl-CoA + 2-acylglycerol = CoA + diacylglycerol. MOGAT2 has a preference toward monoacylglycerols containing unsaturated fatty acids. MOGAT2 plays a central role in absorption of dietary fat in the small intestine by catalyzing the resynthesis of triacylglycerol in enterocytes. And it plays a role in diet-induced obesity. The antibody can recognize all these 3 isoform of MOGAT2.

Publications

Species	Application	Title
mouse	WB	Cell Metab; Adipocyte iron levels impinge on a fat-gut crosstalk to regulate intestinal lipid absorption and mediate protection from obesity.; Authors - Zhuzhen Zhang
mouse	WB	Nat Cell Biol; AIDA directly connects sympathetic innervation to adaptive thermogenesis by UCP1.; Authors - Meng Shi
mouse	WB	Cell Metab; AIDA Selectively Mediates Downregulation of Fat Synthesis Enzymes by ERAD to Retard Intestinal Fat Absorption and Prevent Obesity.; Authors - Hui Luo
human	WB	Cell Res; DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling.; Authors - Pengli Zheng
mouse	WB	Nat Commun; Deficiency of intestinal Bmal1 prevents obesity induced by high-fat feeding.; Authors - Fangjun Yu
pig	WB	Metabolites; Decreased Hyocholic Acid and Lysophosphatidylcholine Induce Elevated Blood Glucose in a Transgenic Porcine Model of Metabolic Disease; Authors - Jianping Xu