Anticorps Polyclonal de lapin anti-MRP2
MRP2 Polyclonal Antibody for IHC, WB,ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain et plus (2)
Applications
WB, IHC, ELISA
Conjugaison
Non conjugué
N° de cat : 24893-1-AP
Synonymes
Galerie de données de validation
Applications testées
| Résultats positifs en WB | cellules HepG2, |
| Résultats positifs en IHC | tissu d'intestin grêle humain il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0. |
Dilution recommandée
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochimie (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Applications publiées
| WB | See 21 publications below |
| IHC | See 2 publications below |
Informations sur le produit
24893-1-AP cible MRP2 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain
| Réactivité | Humain |
| Réactivité citée | rat, Humain, singe |
| Hôte / Isotype | Lapin / IgG |
| Clonalité | Polyclonal |
| Type | Anticorps |
| Immunogène | MRP2 Protéine recombinante Ag18694 |
| Nom complet | ATP-binding cassette, sub-family C (CFTR/MRP), member 2 |
| Masse moléculaire calculée | 1545 aa, 174 kDa |
| Poids moléculaire observé | 190-250 kDa |
| Numéro d’acquisition GenBank | BC136419 |
| Symbole du gène | ABCC2 |
| Identification du gène (NCBI) | 1244 |
| Conjugaison | Non conjugué |
| Forme | Liquide |
| Méthode de purification | Purification par affinité contre l'antigène |
| Tampon de stockage | PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3 |
| Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
Multi-drug resistance protein 2 (MRP2), also known as ABCC2, is an ATP binding cassette (ABC) transporter responsible for biliary excretion of xenobiotics, endobiotics, and their metabolites. Deficiency in ABCC2 results in the clinical disorder Dubin-Johnson syndrome. MRP2 is found to be expressed in a variety of human cancers, and is associated with resistance of tumor cells to various anticancer drugs including cisplatin. The predicted molecular weight of MRP2 is 174 kDa, while mature MRP2 usually has a slower migration around 190-250 kDa due to the glycosylation. (16434545, 18834541, 23045960)
Protocole
| Product Specific Protocols | |
|---|---|
| WB protocol for MRP2 antibody 24893-1-AP | Download protocol |
| IHC protocol for MRP2 antibody 24893-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
ACS Nano Variability in the Clearance of Lead Oxide Nanoparticles Is Associated with Alteration of Specific Membrane Transporters. | ||
Theranostics Circadian Clock Gene Bmal1 Regulates Bilirubin Detoxification: A Potential Mechanism of Feedback Control of Hyperbilirubinemia. | ||
Theranostics Generation of hepatic spheroids using human hepatocyte-derived liver progenitor-like cells for hepatotoxicity screening. | ||
Cell Death Dis MUC1 induces acquired chemoresistance by upregulating ABCB1 in EGFR-dependent manner. | ||
Oncol Res The AKT/GSK3-Mediated Slug Expression Contributes to Oxaliplatin Resistance in Colorectal Cancer via Upregulation of ERCC1. | ||
Sci Rep Functional human induced hepatocytes (hiHeps) with bile acid synthesis and transport capacities: A novel in vitro cholestatic model. |
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH SHENG-FU (Verified Customer) (01-15-2018) | there were multiple bands on the membrane, and the band under 130 kDa were a high expression. But around 190 kDa -250 kDa there are a single band which might be the correct one.
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