Anticorps Polyclonal de lapin anti-Marcksl1

• Phare
• Validé par KD/KO

Marcksl1 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 10002-2-AP

Synonymes

AL022768, AW215397, AW536807, D4Bc1, F52, MacMARCKS, Macs 2, Macs 3, Macs2, Macs3, MARCKS like 1, Marcksl1, Mlp, Mrp

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Galerie de données de validation

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  WB analysis of mouse brain using 10002-2-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10002-2-AP (Marcksl1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 4 publications below
• IF: See 2 publications below

Informations sur le produit

10002-2-AP cible Marcksl1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: MARCKS-like 1
• Masse moléculaire calculée: 40 kDa
• Poids moléculaire observé: 60 kDa
• Numéro d’acquisition GenBank: BC006757
• Symbole du gène: Marcksl1
• Identification du gène (NCBI): 17357
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MacMARCKS is a member of MARCKS family of PKC substrate. It is widely used in the signal transduction studies as an indictor of PKC activation. The protein has a calculated molecular weight of 20 kDa and an apparent molecular weight of 42-45 kDa in different species on SDS PAGE. MacMARCKS functions in many aspects of cell physiology including integrin activation, cell spreading and phagocytosis. Its functions are though to associate with its ability in binding and regulating actin and microtubule cytoskeleton. Current evidence indicated that this protein also involved controls membrane fluidity and molecular mobility of membrane proteins

Publications

Species	Application	Title
mouse	IHC	J Exp Med; Development of intestinal M cells and follicle-associated epithelium is regulated by TRAF6-mediated NF-κB signaling.; Authors - Takashi Kanaya
human	WB,IHC	J Biol Chem; The long noncoding RNA lncZic2 drives the self-renewal of liver tumor-initiating cells via the protein kinase C substrates MARCKS and MARCKSL1.; Authors - Zhenzhen Chen ; KO Validated
human,mouse	WB,IHC	Mol Cell Biol; c-Jun N-terminal kinase phosphorylation of MARCKSL1 determines actin stability and migration in neurons and in cancer cells.; Authors - Bj?rkblom Benny B ; KD Validated
mouse	WB	PLoS One; Colonic miRNA Expression/Secretion, Regulated by Intestinal Epithelial PepT1, Plays an Important Role in Cell-to-Cell Communication during Colitis.; Authors - Saravanan Ayyadurai
human	WB,IF	Exp Cell Res; The regulation of MacMARCKS expression by integrin beta3.; Authors - van den Bout Iman I
human	IHC	Clin Exp Metastasis; Identification of estrogen-responsive genes involved in breast cancer metastases to the bone.; Authors - Wang Jun J