Anticorps Polyclonal de lapin anti-NUP85

NUP85 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(9)

N° de cat : 19370-1-AP

Synonymes

85 kDa nucleoporin, FROUNT, nucleoporin 85kDa, Nucleoporin Nup75, Nucleoporin Nup85, Nup75, NUP85

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Galerie de données de validation

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  WB analysis of mouse lung using 19370-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 19370-1-AP (NUP85 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 19370-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 19370-1-AP (NUP85 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 19370-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 19370-1-AP (NUP85 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 19370-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19370-1-AP (NUP85 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 19370-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach using 19370-1-AP (NUP85 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human stomach using 19370-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach using 19370-1-AP (NUP85 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver using 19370-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 19370-1-AP (NUP85 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 19370-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 19370-1-AP (NUP85 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of hTERT-RPE1 cells using 19370-1-AP

  IF result (Nucleus stain) of anti-NUP85 (19370-1-AP; 1:50) hTERT-RPE1 cell (MeOH fixed) by Dr. Moshe Kim.

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Applications testées

• Résultats positifs en WB: tissu pulmonaire de souris, cellules HeLa, tissu cérébral humain, tissu de thymus de souris
• Résultats positifs en IHC: tissu d'estomac humain, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules hTERT-RPE1

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2400
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IF: See 2 publications below

Informations sur le produit

19370-1-AP cible NUP85 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NUP85 Protéine recombinante Ag7086
• Nom complet: nucleoporin 85kDa
• Masse moléculaire calculée: 75 kDa
• Poids moléculaire observé: 70-75 kDa
• Numéro d’acquisition GenBank: BC000697
• Symbole du gène: NUP85
• Identification du gène (NCBI): 79902
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
		Cell; Stress Granule Assembly Disrupts Nucleocytoplasmic Transport.; Authors - Ke Zhang
human	WB	Oncogene; DNA damage-induced translocation of mitochondrial factor HIGD1A into the nucleus regulates homologous recombination and radio/chemo-sensitivity.; Authors - Bin Chen
human	WB	Genomics Proteomics Bioinformatics; Integrative Multi-omics Landscape of Non-structural Protein 3 of Severe Acute Respiratory Syndrome Coronaviruses.; Authors - Ruona Shi
human	WB	Hum Mol Genet; Expanding the phenotype of NUP85 mutations beyond nephrotic syndrome to primary autosomal recessive microcephaly and Seckel syndrome spectrum disorders.; Authors - Ethiraj Ravindran
human	WB,IF	Front Microbiol; Nucleoporin 85 interacts with influenza A virus PB1 and PB2 to promote its replication by facilitating nuclear import of ribonucleoprotein; Authors - Yue-Huan Ling ; KD Validated
human		bioRxiv; Enterovirus D68 2A protease causes nuclear pore complex dysfunction and motor neuron toxicity; Authors - Katrina M Zinn

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