Anticorps Polyclonal de lapin anti-ORAI1

• Phare
• Validé par KD/KO

ORAI1 Polyclonal Antibody for IF, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications

42

N° de cat : 13130-1-AP

Synonymes

CRACM1, ORAI1 L1, ORAI1 L2, Orail L2, ORAT1, Protein orai 1, TMEM142A, Transmembrane protein 142A

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Galerie de données de validation

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  WB analysis of Jurkat using 13130-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13130-1-AP (ORAI1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 13130-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 13130-1-AP (ORAI1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse ovary using 13130-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 13130-1-AP (ORAI1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 13130-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 13130-1-AP (ORAI1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 13130-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 13130-1-AP (ORAI1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 13130-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13130-1-AP (ORAI1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HEK-293 using 13130-1-AP

  Immunofluorescent analysis of HEK-293 cells using 13130-1-AP (ORAI1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules A375, cellules PC-3, tissu ovarien de souris, tissu splénique de souris
• Résultats positifs en IF: cellules HEK-293

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 34 publications below
• IF: See 8 publications below

Informations sur le produit

13130-1-AP cible ORAI1 dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, poisson-zèbre, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ORAI1 Protéine recombinante Ag3758
• Nom complet: ORAI calcium release-activated calcium modulator 1
• Masse moléculaire calculée: 301 aa, 33 kDa
• Poids moléculaire observé: 45-50 kDa
• Numéro d’acquisition GenBank: BC015369
• Symbole du gène: ORAI1
• Identification du gène (NCBI): 84876
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Store-operated calcium channels (SOCs) play essential functions in various physiological processes. Orai1 (also named CRACM1) is a critical molecular component of SOCs. Orai1 protein is a 32-55 kDa plasma membrane protein with 4 transmembrane domains. Human patients with a severe combinaed immunodeficiency (SCID) syndrome possess point mutations in Orai1 gene.

Publications

Species	Application	Title
human	WB,IF	Cell Res; Direct control of store-operated calcium channels by ultrafast laser.; Authors - Pan Cheng ; KO Validated
mouse	WB	Blood; Orai1 (CRACM1) is the platelet SOC channel and essential for pathological thrombus formation.; Authors - Braun Attila A
mouse	WB	Blood; The serum- and glucocorticoid-inducible kinase 1 (SGK1) influences platelet calcium signaling and function by regulation of Orai1 expression in megakaryocytes.; Authors - Borst Oliver O
mouse	WB	Kidney Int; AMP-activated kinase is a regulator of fibroblast growth factor 23 production.; Authors - Philipp Glosse
mouse	IF	Sci Signal; Adhesion to laminin-1 and collagen IV induces the formation of Ca2+ microdomains that sensitize mouse T cells for activation; Authors - Mariella Weiß
mouse	IF	Sci Signal; ORAI1, STIM1/2, and RYR1 shape subsecond Ca2+ microdomains upon T cell activation.; Authors - Björn-Philipp Diercks

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