Anticorps Polyclonal de lapin anti-PDK1

PDK1 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

25

N° de cat : 10026-1-AP

Synonymes

PDH kinase 1, PDHK1, PDK1, Pyruvate dehydrogenase kinase isoform 1

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Galerie de données de validation

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  WB analysis of mouse liver using 10026-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10026-1-AP (PDK1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse small intestine using 10026-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 10026-1-AP (PDK1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 10026-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10026-1-AP (PDK1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of NIH/3T3 using 10026-1-AP

  IP Result of anti-PDK1 (IP:10026-1-AP, 6ug; Detection:10026-1-AP 1:1000) with NIH/3T3 cells lysate 1200ug.

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  IHC staining of human small intestine using 10026-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 10026-1-AP (PDK1 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human small intestine using 10026-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 10026-1-AP (PDK1 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human colon using 10026-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10026-1-AP (PDK1 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon using 10026-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10026-1-AP (PDK1 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human liver using 10026-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 10026-1-AP (PDK1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 10026-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 10026-1-AP (PDK1 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris, cellules NIH/3T3, tissu d'intestin grêle de souris
• Résultats positifs en IP: cellules NIH/3T3
• Résultats positifs en IHC: tissu d'intestin grêle humain, tissu de côlon humain, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 20 publications below
• IHC: See 3 publications below

Informations sur le produit

10026-1-AP cible PDK1 dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PDK1 Protéine recombinante Ag0025
• Nom complet: pyruvate dehydrogenase kinase, isozyme 1
• Masse moléculaire calculée: 436 aa, 49 kDa
• Poids moléculaire observé: 46-55 kDa
• Numéro d’acquisition GenBank: BC039158
• Symbole du gène: PDK1
• Identification du gène (NCBI): 5163
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PDK1(pyruvate dehydrogenase kinase isoform 1) is also named as PDHK1 and belongs to the PDK/BCKDK protein kinase family. It inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism (PMID:17683942). Starvation increases islet PDK activity (PMID:11723055). PDK1 has 2 isoforms with the MW of 49 and 52 kD, and a 28aa-transit-peptide in the N-terminal can be removed in the mature form. This antibody is speicfic to PDK1.

Publications

Species	Application	Title
human	WB	Proc Natl Acad Sci U S A; LncRNA IDH1-AS1 links the functions of c-Myc and HIF1α via IDH1 to regulate the Warburg effect.; Authors - Shaoxun Xiang
human	WB	EMBO Rep; CUE domain-containing protein 2 promotes the Warburg effect and tumorigenesis.; Authors - Xiuying Zhong
mouse	WB	Front Endocrinol (Lausanne); HIF-1α Regulates Glucocorticoid-Induced Osteoporosis Through PDK1/AKT/mTOR Signaling Pathway.; Authors - Wen-Ning Xu
		Cells; Prox1 Suppresses the Proliferation of Breast Cancer Cells via Direct Inhibition of c-Myc Gene Expression; Authors - Artemis Michail
rat	WB	J Cell Mol Med; PMID: 31945804; Authors - Chenyang Chen
human	IHC	Front Oncol; Overexpression of Pyruvate Dehydrogenase Kinase-3 Predicts Poor Prognosis in Urothelial Carcinoma; Authors - Yu-Hsuan Kuo