Anticorps Polyclonal de lapin anti-Twinkle/PEO1

Twinkle/PEO1 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(7)

N° de cat : 13435-1-AP

Synonymes

Twinkle, PEO1, PEO, IOSCA, EC:5.6.2.3

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Galerie de données de validation

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  WB analysis of HeLa using 13435-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13435-1-AP (Twinkle antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 13435-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13435-1-AP (Twinkle antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse skeletal muscle using 13435-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 13435-1-AP (Twinkle antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 13435-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 13435-1-AP (Twinkle antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules Jurkat
• Résultats positifs en IHC: tissu de muscle squelettique de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below

Informations sur le produit

13435-1-AP cible Twinkle/PEO1 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Twinkle/PEO1 Protéine recombinante Ag4239
• Nom complet: chromosome 10 open reading frame 2
• Masse moléculaire calculée: 684 aa, 77 kDa
• Poids moléculaire observé: 60 kDa
• Numéro d’acquisition GenBank: BC033762
• Symbole du gène: PEO1
• Identification du gène (NCBI): 56652
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Twinkle, also named as C10orf2, PEO1, is a 684 amino acid protein, which forms multimers in vitro, including hexamers. Twinkle colocalizes with mtDNA in mitochondrial nucleoids, a nucleoproteins complex consisting of a number of copies of proteins associated with mtDNA, probably involved in mtDNA maintenance and expression. Twinkle involves in mitochondrial DNA (mtDNA) metabolism and could function as an adenine nucleotide-dependent DNA helicase. Twinkle exists various isoform and molecular weight of isoform is 60 kDa.

Publications

Species	Application	Title
mouse	WB	Nat Commun; Mitofusin 1 and 2 regulation of mitochondrial DNA content is a critical determinant of glucose homeostasis.; Authors - Vaibhav Sidarala
human		EMBO J; ATAD3B is a mitophagy receptor mediating clearance of oxidative stress-induced damaged mitochondrial DNA.; Authors - Li Shu
human	WB	Cell Rep; Sam50 Regulates PINK1-Parkin-Mediated Mitophagy by Controlling PINK1 Stability and Mitochondrial Morphology.; Authors - Fenglei Jian
mouse	WB	Cell Death Differ; Mic60/Mitofilin determines MICOS assembly essential for mitochondrial dynamics and mtDNA nucleoid organization.; Authors - H Li
mouse	WB	Sci Rep; PMID: 28397817; Authors - Xinbin Zhao
human	WB	Nucleic Acids Res; A deafness-associated mitochondrial DNA mutation caused pleiotropic effects on DNA replication and tRNA metabolism; Authors - Feilong Meng

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