Anticorps Polyclonal de lapin anti-PI3 Kinase p110 Beta

PI3 Kinase p110 Beta Polyclonal Antibody for IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(19)

N° de cat : 21739-1-AP

Synonymes

p110 BETA, PI3 Kinase p110 Beta, PI3 kinase subunit beta, PI3K, PI3K beta, PI3K p110(beta), PI3Kbeta, PI3KCB, PIK3C1, PIK3CB, PtdIns 3 kinase subunit beta

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Galerie de données de validation

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  IHC staining of human liver cancer using 21739-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 21739-1-AP (PI3K p110(beta) antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver cancer using 21739-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 21739-1-AP (PI3K p110(beta) antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 21739-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 21739-1-AP (PI3K p110(beta) antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 21739-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 21739-1-AP (PI3K p110(beta) antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 21739-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 21739-1-AP (PI3K p110(beta) antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en IHC: tissu de cancer du foie humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 16 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

21739-1-AP cible PI3 Kinase p110 Beta dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PI3 Kinase p110 Beta Protéine recombinante Ag16456
• Nom complet: phosphoinositide-3-kinase, catalytic, beta polypeptide
• Masse moléculaire calculée: 1070 aa, 123 kDa
• Poids moléculaire observé: 110 kDa
• Numéro d’acquisition GenBank: BC114432
• Symbole du gène: PI3 Kinase p110 Beta
• Identification du gène (NCBI): 5291
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PIK3CB(phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform) is also named as PIK3C1, PI3K-beta, p110beta. The gene encodes a 1070 amino acid protein which belongs to the PI3/PI4-kinase family. Phosphoinositide 3-kinases (PI3Ks) have been implicated as participants in signaling pathways regulating cell growth by virtue of their activation in response to various mitogenic stimuli. The class I PI3 kinases are heterodimers composed of 110 kDa catalytic subunits that associate with regulatory adaptor proteins. Four class I catalytic subunits have been identified, PIK3CA (p110α), PIK3CB (p110β), PIK3CD (p110δ) and PIK3CG (p110γ)(PMID:19177002).

Publications

Species	Application	Title
human	WB	J Adv Res; Salidroside sensitizes Triple-negative breast cancer to ferroptosis by SCD1-mediated lipogenesis and NCOA4-mediated ferritinophagy; Authors - Guiqin Huang
human,mouse	WB	Cell Mol Gastroenterol Hepatol; Mannan-binding lectin via interaction with cell surface calreticulin promotes senescence of activated hepatic stellate cells to limit liver fibrosis progression.; Authors - Jialiang Luo
human	WB	Biochem Pharmacol; TANGO1 interacts with NRTN to promote hepatocellular carcinoma progression by regulating the PI3K/AKT/mTOR signaling pathway; Authors - Jing Man
human	WB	J Ethnopharmacol; Root extract of Hemsleya amabilis Diels suppresses renal cell carcinoma cell growth through inducing apoptosis and G2/M phase arrest via PI3K/AKT signaling pathway; Authors - Kai Li
human	WB	Cells; Determination of WWOX Function in Modulating Cellular Pathways Activated by AP-2α and AP-2γ Transcription Factors in Bladder Cancer.; Authors - Damian Kołat
human	WB	J Zhejiang Univ Sci B; Silencing of DsbA-L gene impairs the PPARγ agonist function of improving insulin resistance in a high-glucose cell model.; Authors - Xuan Zhou

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