Anticorps Polyclonal de lapin anti-RB1

RB1 Polyclonal Antibody for WB, IP, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IP, IF, ELISA

Conjugaison

Non conjugué

Publications(5)

N° de cat : 25628-1-AP

Synonymes

OSRC, p105 Rb, pp110, pRb, RB, RB1, retinoblastoma 1

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Galerie de données de validation

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  WB analysis of Jurkat using 25628-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 25628-1-AP (RB1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 25628-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 25628-1-AP (RB1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 25628-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 25628-1-AP (RB1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 25628-1-AP

  IP result of anti-RB1 (IP:25628-1-AP, 4ug; Detection:25628-1-AP 1:300) with Jurkat cells lysate 3400ug.

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  IF Staining of HepG2 using 25628-1-AP

  Immunofluorescent analysis of HepG2 cells using 25628-1-AP (RB1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules A431
• Résultats positifs en IP: cellules Jurkat
• Résultats positifs en IF/ICC: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IF: See 1 publications below

Informations sur le produit

25628-1-AP cible RB1 dans les applications de WB, IP, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RB1 Protéine recombinante Ag22578
• Nom complet: retinoblastoma 1
• Masse moléculaire calculée: 928 aa, 106 kDa
• Poids moléculaire observé: 110 kDa
• Numéro d’acquisition GenBank: BC039060
• Symbole du gène: RB1
• Identification du gène (NCBI): 5925
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Artif Cells Nanomed Biotechnol; Ribociclib (LEE011) suppresses cell proliferation and induces apoptosis of MDA-MB-231 by inhibiting CDK4/6-cyclin D-Rb-E2F pathway.; Authors - Tianqi Li
human	WB	Oncol Rep; Actin-capping protein CapG is associated with prognosis, proliferation and metastasis in human glioma.; Authors - Da-Peng Yun
human	IF	Oncol Lett; 14-3-3ε is a nuclear matrix protein, and its altered expression and localization are associated with curcumin-induced apoptosis of MG-63 cells.; Authors - Kun Lu
human	WB	Cell Death Dis; MCPIP1-mediated NFIC alternative splicing inhibits proliferation of triple-negative breast cancer via cyclin D1-Rb-E2F1 axis.; Authors - Fengxia Chen
human	WB	Biomed Pharmacother; Ribociclib, a selective cyclin D kinase 4/6 inhibitor, inhibits proliferation and induces apoptosis of human cervical cancer in vitro and in vivo.; Authors - Yudi Xiong

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