Anticorps Monoclonal anti-RXRA

RXRA Monoclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(2)

CloneNo.

4H6C4

N° de cat : 60198-1-Ig

Synonymes

NR2B1, Retinoid X receptor alpha, retinoid X receptor, alpha, RXRA, RXRα

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Galerie de données de validation

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  WB analysis of HeLa using 60198-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 60198-1-Ig (RXRA antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach cancer using 60198-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60198-1-Ig (RXRA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 60198-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 60198-1-Ig (RXRA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat liver using 60198-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 60198-1-Ig (RXRA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 60198-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60198-1-Ig (RXRA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 60198-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 60198-1-Ig (RXRA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat liver using 60198-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 60198-1-Ig (RXRA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa
• Résultats positifs en IHC: tissu de cancer de l'estomac humain, tissu de cervelet de souris, tissu hépatique de rat; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

60198-1-Ig cible RXRA dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: RXRA Protéine recombinante Ag0987
• Nom complet: retinoid X receptor, alpha
• Masse moléculaire calculée: 462 aa, 51 kDa
• Poids moléculaire observé: 44 kDa
• Numéro d’acquisition GenBank: BC007925
• Symbole du gène: RXRA
• Identification du gène (NCBI): 6256
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Retinoid X receptor alpha (RXRA). Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. The high-affinity ligand for RXRs is 9-cis retinoic acid. RXRA serves as a common heterodimeric partner for a number of nuclear receptors. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence of a ligand, the RXR-RAR heterodimers associate with a multiprotein complex containing transcription corepressors that induce histone acetylation, chromatin condensation, and transcriptional suppression. On ligand binding, the corepressors dissociate from the receptors and associate with the coactivators leading to transcriptional activation. The RXRA/PPARA heterodimer is required for PPARA transcriptional activity on fatty acid oxidation genes such as ACOX1 and the P450 system genes. This antibody is a rabbit polyclonal antibody raised against the 350 AA of human RXRA C-terminal. RXRA is highly expressed in the liver, and also expressed in the lungs, kidneys, and heart. It can recognize the the mature 54 kDa RXRA and the truncated 44 kD RXRA (PMID: 20541701).

Publications

Species	Application	Title
human	WB	Int J Endocrinol; Constitutive Androstane Receptor-Mediated Inhibition of Metformin on Phase II Metabolic Enzyme SULT2A1; Authors - Xiaowen Hu
mouse	WB	Exp Mol Med; LncRNA Gm35585 transcriptionally activates the peroxidase EHHADH against diet-induced fatty liver; Authors - Ming Jin

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