Anticorps Polyclonal de lapin anti-SAR1A

• Phare
• Validé par KD/KO

SAR1A Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 15350-1-AP

Synonymes

COPII associated small GTPase, GTP binding protein SAR1a, masra2, SAR1, SAR1 homolog A (S. cerevisiae), SAR1A, Sara, SARA1

---

Galerie de données de validation

• 

  WB analysis of mouse liver using 15350-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15350-1-AP (SAR1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 15350-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 15350-1-AP (SAR1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 15350-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 15350-1-AP (SAR1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15350-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15350-1-AP (SAR1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 15350-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15350-1-AP (SAR1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human hepatocirrhosis using 15350-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 15350-1-AP (SAR1A antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human hepatocirrhosis using 15350-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 15350-1-AP (SAR1A antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human liver using 15350-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15350-1-AP (SAR1A Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 15350-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15350-1-AP (SAR1A Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skeletal muscle using 15350-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 15350-1-AP (SAR1A Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 15350-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 15350-1-AP (SAR1A Antibody) at dilution of 1:50 (under 40x lens).

---

Applications testées

• Résultats positifs en WB: tissu hépatique de souris, cellules HeLa, cellules HT-1080
• Résultats positifs en IHC: tissu de cirrhose hépatique humain, tissu de muscle squelettique humain, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

15350-1-AP cible SAR1A dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SAR1A Protéine recombinante Ag7596
• Nom complet: SAR1 homolog A (S. cerevisiae)
• Masse moléculaire calculée: 22 kDa
• Poids moléculaire observé: 23-25 kDa
• Numéro d’acquisition GenBank: BC003658
• Symbole du gène: SAR1A
• Identification du gène (NCBI): 56681
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB	J Clin Invest; Sec13 promotes oligodendrocyte differentiation and myelin repair through autocrine pleiotrophin signaling.; Authors - Zhixiong Liu
human,mouse	WB	Redox Biol; Selenoprotein K contributes to CD36 subcellular trafficking in hepatocytes by accelerating nascent COPII vesicle formation and aggravates hepatic steatosis; Authors - Mengyue You
human	WB	Proc Natl Acad Sci U S A; Atlastin-mediated membrane tethering is critical for cargo mobility and exit from the endoplasmic reticulum.; Authors - Liling Niu
rat	WB	J Biol Chem; The Endoplasmic Reticulum COPII-Vesicle Transport Machinery Coordinates Cellular Lipid Secretion and Cholesterol Biosynthesis.; Authors - Lee G D Fryer
human,mouse	IHC,WB	Clin Epigenetics; Epigenetic silencing of TMEM176A activates ERK signaling in human hepatocellular carcinoma.; Authors - Hongxia Li ; KD Validated
human	WB,IP	Biochem Biophys Res Commun; Pull down assay for GTP-bound form of Sar1a reveals its activation during morphological differentiation.; Authors - Yuri Urai