Anticorps Polyclonal de lapin anti-SCD

• Phare
• Validé par KD/KO

SCD Polyclonal Antibody for IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat et plus (1)

Applications

IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

36

N° de cat : 23393-1-AP

Synonymes

Acyl CoA desaturase, Delta(9) desaturase, FADS5, Fatty acid desaturase, MSTP008, SCD, SCD1, SCDOS, Stearoyl CoA desaturase

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Galerie de données de validation

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  IHC staining of human liver using 23393-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 23393-1-AP (SCD Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human brain using 23393-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 23393-1-AP (SCD Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human brain using 23393-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 23393-1-AP (SCD Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human brain using 23393-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 23393-1-AP (SCD Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human brain using 23393-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 23393-1-AP (SCD Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human liver using 23393-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 23393-1-AP (SCD Antibody) at dilution of 1:200 (under 10x lens).

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Applications testées

• Résultats positifs en IHC: tissu hépatique humain, tissu cérébral humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• IHC: See 10 publications below
• IF: See 2 publications below

Informations sur le produit

23393-1-AP cible SCD dans les applications de IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SCD Protéine recombinante Ag13456
• Nom complet: stearoyl-CoA desaturase (delta-9-desaturase)
• Masse moléculaire calculée: 355 aa, 41 kDa
• Poids moléculaire observé: 40 kDa
• Numéro d’acquisition GenBank: BC005807
• Symbole du gène: SCD
• Identification du gène (NCBI): 6319
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SCD (stearoyl-CoA desaturase) is a 37-45 kDa (PMID:10946019) microsomal fatty acid monodesaturase, also commonly known as 9-desaturase, which catalyses the committed step in the biosynthesis of mono-unsaturated fatty acids from saturated fatty acids. Five SCD genes (SCD1, SCD2, SCD3,SCD4 and SCD5) have been identified and characterized in mice and SCD1 and SCD2 are the main isoforms expressed in mouse liver and brain respectively, SCD3 is expressed exclusively in skin, whereas SCD4 is expressed predominantly in the heart. SCD1and SCD5 has been the only human SCD gene identified and characterized todate(PMID:15907797 ). The formation of homodimers and oligomers is an intrinsic property of SCD proteins, which may play an important role in regulating the half-life of the SCDenzymes, thus representing a novel regulatory mechanism for SCD enzymes, in addition to the transcriptional and post-translational regulations. A 32-kDa SCD1 degradation product identified may be caused by a major cleavage site at the C-terminus, thus representing a novel degradation product(PMID:15610069).

Publications

Species	Application	Title
human		Nucleic Acids Res; EWS-FLI1 regulates and cooperates with core regulatory circuitry in Ewing sarcoma.; Authors - Xianping Shi
human		EMBO J; A stress-induced tyrosine-tRNA depletion response mediates codon-based translational repression and growth suppression.; Authors - Doowon Huh
human,mouse	IHC	Stem Cells; Increased lipogenesis is critical for self-renewal and growth of breast cancer stem cells: Impact of omega-3 fatty acids.; Authors - Haiqing Luo ; KD Validated
human		Liver Int; Disruption of Plin5 degradation by CMA causes lipid homeostasis imbalance in NAFLD.; Authors - Shuo Y Ma
mouse		J Physiol; Sex-specific metabolic responses to 6 hours of fasting during the active phase in young mice.; Authors - Therese Freire
human		Int J Biol Sci; Long noncoding RNA SH3PXD2A-AS1 promotes colorectal cancer progression by regulating p53-mediated gene transcription.; Authors - Pingfu Hou