Anticorps Polyclonal de lapin anti-SLC7A5

• Phare
• Validé par KD/KO

SLC7A5 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

16

N° de cat : 13752-1-AP

Synonymes

4F2 light chain, 4F2LC, CD98 light chain, CD98LC, D16S469E, E16, hLAT1, Integral membrane protein E16, LAT1, MPE16, SLC7A5

---

Galerie de données de validation

• 

  WB analysis of mouse lung using 13752-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 13752-1-AP (SLC7A5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 13752-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 13752-1-AP (SLC7A5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 13752-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 13752-1-AP (SLC7A5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung using 13752-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 13752-1-AP (SLC7A5 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 13752-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 13752-1-AP (SLC7A5 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human stomach cancer using 13752-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 13752-1-AP (SLC7A5 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human stomach cancer using 13752-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 13752-1-AP (SLC7A5 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 13752-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 13752-1-AP (SLC7A5 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 13752-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 13752-1-AP (SLC7A5 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse lung using 13752-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 13752-1-AP (SLC7A5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 13752-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 13752-1-AP (SLC7A5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 13752-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 13752-1-AP (SLC7A5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 13752-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 13752-1-AP (SLC7A5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 13752-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 13752-1-AP (SLC7A5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Applications testées

• Résultats positifs en WB: tissu pulmonaire de souris, cellules MCF-7, tissu placentaire humain
• Résultats positifs en IHC: tissu pulmonaire humain, tissu de cancer de l'estomac humain, tissu hépatique de souris, tissu pulmonaire de souris, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 11 publications below
• IHC: See 6 publications below
• IF: See 4 publications below

Informations sur le produit

13752-1-AP cible SLC7A5 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris, Vache
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SLC7A5 Protéine recombinante Ag4694
• Nom complet: solute carrier family 7 (cationic amino acid transporter, y+ system), member 5
• Masse moléculaire calculée: 507 aa, 55 kDa
• Poids moléculaire observé: 45-50 kDa
• Numéro d’acquisition GenBank: BC039692
• Symbole du gène: SLC7A5
• Identification du gène (NCBI): 8140
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Cell Mol Life Sci; Regulation of branched-chain amino acid metabolism by hypoxia-inducible factor in glioblastoma.; Authors - Bo Zhang
human	IHC	J Nucl Med; PMID: 29700126; Authors - Deep Hathi
mouse	WB	Mol Metab; Resistance exercise enhances long-term mTORC1 sensitivity to leucine; Authors - Gommaar D'Hulst
human	WB	ACS Chem Neurosci; Baicalein Delays H2O2-Induced Astrocytic Senescence through Inhibition of Senescence-Associated Secretory Phenotype (SASP), Suppression of JAK2/STAT1/NF-κB Pathway, and Regulation of Leucine Metabolism.; Authors - Li Gao
human	WB,IHC	J Biol Chem; GSE1 predicts poor survival outcome in gastric cancer patients by SLC7A5 enhancement of tumor growth and metastasis.; Authors - Keshuo Ding
human	IHC	Br J Cancer; ASCT2 (SLC1A5)-dependent glutamine uptake is involved in the progression of head and neck squamous cell carcinoma.; Authors - Ze Zhang

Produits associés

CD98/SLC3A2 Polyclonal antibody

15193-1-AP

CD147 Polyclonal antibody

11989-1-AP