Anticorps Polyclonal de lapin anti-SMCR7/MID49

• Phare
• Validé par KD/KO

SMCR7/MID49 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (2)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(54)

Avis (1)

N° de cat : 16413-1-AP

Synonymes

MID49, MIEF2, SMCR7

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Galerie de données de validation

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  WB analysis using 16413-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16413-1-AP (SMCR7/MID49 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293T using 16413-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 16413-1-AP (SMCR7/MID49 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 16413-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 16413-1-AP (SMCR7/MID49 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 16413-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 16413-1-AP (SMCR7/MID49 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 16413-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 16413-1-AP (SMCR7/MID49 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 16413-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16413-1-AP (SMCR7/MID49 antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver cancer using 16413-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16413-1-AP (SMCR7/MID49 antibody at dilution of 1:50 (under 40x lens).

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  IHC staining of human skeletal muscle using 16413-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 16413-1-AP (SMCR7/MID49 antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human skeletal muscle using 16413-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 16413-1-AP (SMCR7/MID49 antibody at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HEK-293T, cellules HepG2, cellules HT-1080
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu de muscle squelettique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 10 publications below
• WB: See 52 publications below
• IHC: See 3 publications below
• IF: See 3 publications below

Informations sur le produit

16413-1-AP cible SMCR7/MID49 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SMCR7/MID49 Protéine recombinante Ag9531
• Nom complet: Smith-Magenis syndrome chromosome region, candidate 7
• Masse moléculaire calculée: 454 aa, 49 kDa
• Poids moléculaire observé: 49 kDa
• Numéro d’acquisition GenBank: BC014973
• Symbole du gène: MID49
• Identification du gène (NCBI): 125170
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SMCR7, also named as MID49, is a 49 kDa mitochondrial outer membrane protein which regulates mitochondrial morphology. SMCR7 can directly recruit the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface. SMCR7 has two isoforms.

Publications

Species	Application	Title
human		Science; Golgi-derived PI(4)P-containing vesicles drive late steps of mitochondrial division.; Authors - Shun Nagashima
mouse	WB	Cell Metab; Mitochondrial Dynamics Is Critical for the Full Pluripotency and Embryonic Developmental Potential of Pluripotent Stem Cells.; Authors - Xiuying Zhong
monkey	WB	Nat Cell Biol; MIROs and DRP1 drive mitochondrial-derived vesicle biogenesis and promote quality control.; Authors - Tim König ; KO Validated
human	WB	Autophagy; Loss of MIEF1/MiD51 confers susceptibility to BAX-mediated cell death and PINK1-PRKN-dependent mitophagy.; Authors - Hongxu Xian ; KD Validated
mouse	WB,IF	J Cell Biol; The i-AAA protease YME1L and OMA1 cleave OPA1 to balance mitochondrial fusion and fission.; Authors - Ruchika Anand
human	WB	EMBO J; FUNDC1 regulates mitochondrial dynamics at the ER-mitochondrial contact site under hypoxic conditions.; Authors - Wenxian Wu ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Chun (Verified Customer) (07-03-2019)

This antibody is very good.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:2,000
• Cell Tissue Type: HEK293 and HeLa cells

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