Anticorps Polyclonal de lapin anti-TFAM

TFAM Polyclonal Antibody for IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (2)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

40

N° de cat : 19998-1-AP

Synonymes

MtTF1, mtTFA, TCF 6, TCF6, TCF6L1, TCF6L2, TCF6L3, TFAM, Transcription factor 6, Transcription factor 6 like 2

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Galerie de données de validation

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  WB analysis of Jurkat using 19998-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 19998-1-AP (TFAM antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 19998-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 19998-1-AP (TFAM Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 19998-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 19998-1-AP (TFAM antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 19998-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 19998-1-AP (TFAM antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 19998-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 19998-1-AP (TFAM antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 19998-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 19998-1-AP (TFAM antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 19998-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 19998-1-AP (TFAM antibody) at dilution of 1:50 (under 10x lens).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HEK-293
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu cardiaque humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 36 publications below
• IHC: See 3 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Informations sur le produit

19998-1-AP cible TFAM dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, poisson-zèbre, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: transcription factor A, mitochondrial
• Masse moléculaire calculée: 29 kDa
• Numéro d’acquisition GenBank: NM_003201
• Symbole du gène: TFAM
• Identification du gène (NCBI): 7019
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TFAM, also named as TCF6L2, MtTF1, TCF6, TCF6L1, TCF6L3 and mtTFA, is a mitochondrial transcription factor that is a key activator of mitochondrial transcription as well as a participant in mitochondrial genome replication. It is involved in mitochondrial transcription regulation. TFAM is required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. It activates transcription by binding immediately upstream of transcriptional start sites. TFAM is able to unwind and bend DNA. The molecular weighgt of TFAM isoforms are 25 kDa and 29 kDa,but the Ubiquitination of TFAM protein is about 35-37 kDa (PMID: 24607487).

Publications

Species	Application	Title
mouse	WB	Cell; MicroRNA Directly Enhances Mitochondrial Translation during Muscle Differentiation.; Authors - Xiaorong Zhang
human	WB	Nat Immunol; Kinases Mst1 and Mst2 positively regulate phagocytic induction of reactive oxygen species and bactericidal activity.; Authors - Jing Geng
zebrafish	WB	Nucleic Acids Res; Ablation of Mto1 in zebrafish exhibited hypertrophic cardiomyopathy manifested by mitochondrion RNA maturation deficiency.; Authors - Qinghai Zhang
human	WB	Redox Biol; Defective mitochondrial ISCs biogenesis switches on IRP1 to fine tune selective mitophagy.; Authors - Hao Wu
human	WB	Proc Natl Acad Sci U S A; Mitochondrial Nuclear Retrograde Regulator 1 (MNRR1) rescues the cellular phenotype of MELAS by inducing homeostatic mechanisms.; Authors - Siddhesh Aras
mouse,human	IHC,IF	Kidney Int; Kidney epithelial targeted mitochondrial transcription factor A deficiency results in progressive mitochondrial depletion associated with severe cystic disease.; Authors - Ken Ishii ; KO Validated