Anticorps Recombinant de lapin anti-TIGIT

TIGIT Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

FC

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

240242G1

N° de cat : CL488-98026

Synonymes

Vstm3, 240242G1

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Galerie de données de validation

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  FC experiment of mouse splenocytes using CL488-98026

  1x10^6 untreated or anti-CD3/CD28 treated mouse splenocytes were surface stained with CoraLite® Plus 647 Anti-Mouse CD4 and 0.25 ug CoraLite® Plus 488 Anti-Mouse TIGIT Rabbit Recombinant Antibody (CL488-98026, Clone:240242G1). Cells were not fixed.

• 

  FC experiment of mouse splenocytes using CL488-98026

  1x10^6 anti-CD3/CD28 treated mouse splenocytes were surface stained with 0.25 ug CoraLite® Plus 488 Anti-Mouse TIGIT Rabbit Recombinant Antibody (CL488-98026, Clone: 240242G1) (red) or Isotype Control (blue). 1x10^6 untreated mouse splenocytes were surface stained with 0.25ug CoraLite® Plus 488 Anti-Mouse TIGIT Rabbit Recombinant Antibody (CL488-98026, Clone: 240242G1) (black, dashed). Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: Anti-CD3/CD28 treated mouse splenocytes

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-98026 cible TIGIT dans les applications de FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: T cell immunoreceptor with Ig and ITIM domains
• Masse moléculaire calculée: 26kDa
• Numéro d’acquisition GenBank: NP_001139797.1
• Symbole du gène: Tigit
• Identification du gène (NCBI): 100043314
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

TIGIT (T-cell immunoreceptor with Ig and ITIM domains), also known as VSIG9 or VSTM3, is an immune receptor expressed on T cells, including Treg and memory subsets, as well as on NK cells (PMID: 19011627). It contains an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT binds to poliovirus receptor (PVR, also called CD155) with high affinity, and also to PVRL2 (CD112) with lower affinity. The interaction of TIGIT with PVR on dendritic cells increases the secretion of IL-10 and decreases the secretion of proinflammatory cytokine and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells. TIGIT can inhibit NK cytotoxicity directly through its ITIM (PMID: 19815499).

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