Anticorps Polyclonal de lapin anti-TRAF1

TRAF1 Polyclonal Antibody for WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB,ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 11543-1-AP

Synonymes

EBI6, TRAF1

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Galerie de données de validation

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  WB analysis of Jurkat using 11543-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11543-1-AP (TRAF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 11543-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11543-1-AP (TRAF1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules Jurkat

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

11543-1-AP cible TRAF1 dans les applications de WB,ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TRAF1 Protéine recombinante Ag2131
• Nom complet: TNF receptor-associated factor 1
• Masse moléculaire calculée: 416 aa, 46 kDa
• Poids moléculaire observé: 46-50 kDa
• Numéro d’acquisition GenBank: BC024145
• Symbole du gène: TRAF1
• Identification du gène (NCBI): 7185
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TRAF1 is a member of the TNF receptor (TNFR) associated factor (TRAF) protein family. TRAF proteins associate with, and mediate the signal transduction from various receptors of the TNFR superfamily. TRAF1 and TRAF2 form a heterodimeric complex, which is required for TNF-alpha-mediated activation of MAPK8/JNK and NF-kappaB. The protein complex formed by this protein and TRAF2 also interacts with inhibitor-of-apoptosis proteins (IAPs), and thus mediates the anti-apoptotic signals from TNF receptors. The expression of this protein can be induced by Epstein-Barr virus (EBV). EBV infection membrane protein 1 (LMP1) is found to interact with this and other TRAF proteins; this interaction is thought to link LMP1-mediated B lymphocyte transformation to the signal transduction from TNFR family receptors. TRAF1 is observed at 45-50 kDa in western blotting, and the additional bands of lower molecular weight (~30 kDa) may represent TRAF1 cleavage fragments, similar to the results obtained by the article (PMID:11098060).

Publications

Species	Application	Title
mouse	WB	Eur J Nutr; Trans-10-hydroxy-2-decenoic acid protects against LPS-induced neuroinflammation through FOXO1-mediated activation of autophagy.; Authors - Mengmeng You