Anticorps Polyclonal de lapin anti-TREM1

TREM1 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

10

N° de cat : 11791-1-AP

Synonymes

CD354, TREM 1, TREM1

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Galerie de données de validation

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  WB analysis of human peripheral blood leukocyte using 11791-1-AP

  human peripheral blood leukocyte were subjected to SDS PAGE followed by western blot with 11791-1-AP (TREM1 antibody) at dilution of 1:450 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 11791-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 11791-1-AP (TREM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 11791-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 11791-1-AP (TREM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human spleen using 11791-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 11791-1-AP (TREM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 11791-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11791-1-AP (TREM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 11791-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11791-1-AP (TREM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 11791-1-AP

  Immunofluorescent analysis of HepG2 cells, using TREM1 antibody 11791-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).Blue pseudocolor = DAPI (fluorescent DNA dye).

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  FC experiment of THP-1 using 11791-1-AP

  1X10^6 THP-1 cells were intracellularly stained with 0.4 ug Anti-Human TREM1 (11791-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: leucocyte de sang périphérique humain, tissu hépatique humain, tissu pulmonaire de souris, tissu splénique humain
• Résultats positifs en IHC: tissu d'amygdalite humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2
• Résultats positifs en cytométrie: cellules THP-1,

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below
• IHC: See 2 publications below
• IF: See 4 publications below
• FC: See 1 publications below

Informations sur le produit

11791-1-AP cible TREM1 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TREM1 Protéine recombinante Ag2418
• Nom complet: triggering receptor expressed on myeloid cells 1
• Masse moléculaire calculée: 234 aa, 26 kDa
• Poids moléculaire observé: 30 kDa
• Numéro d’acquisition GenBank: BC017773
• Symbole du gène: TREM1
• Identification du gène (NCBI): 54210
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Triggering receptor expressed on myeloid cells 1 (TREM1, also known as CD354) is a member of the immunoglobulin superfamily, which is expressed on neutrophils and monocytes. TREM1 plays a role in the innate immune system. TREM1 amplifies neutrophil and monocyte-mediated inflammatory responses triggered by bacterial and fungal infections by stimulating release of pro-inflammatory chemokines and cytokines, as well as increased surface expression of cell activation markers.

Publications

Species	Application	Title
mouse	WB	Theranostics; A COX-2/sEH dual inhibitor PTUPB alleviates lipopolysaccharide-induced acute lung injury in mice by inhibiting NLRP3 inflammasome activation.; Authors - Hui-Hui Yang
mouse	WB	Int Immunopharmacol; Activation of NLRP3 inflammasome up-regulates TREM-1 expression in murine macrophages via HMGB1 and IL-18.; Authors - Wen-Jing Zhong
mouse	IF	Acta Trop; Malaria-derived exosomes exacerbate liver injury during blood stage of Plasmodium berghei infection; Authors - Xin Zhang
human	WB,IHC	J Inflamm Res; Overexpression of TREM1 is Associated with the Immune-Suppressive Microenvironment and Unfavorable Prognosis in Pan-Cancer; Authors - Xinwei Zhou
human	IF	Neurol Neuroimmunol Neuroinflamm; Jo-1 Antibodies From Myositis Induce Complement-Dependent Cytotoxicity and TREM-1 Upregulation in Muscle Endothelial Cells; Authors - Masaya Honda
mouse	IF,WB	Behav Brain Res; Inhibition of TREM-1 Ameliorates Lipopolysaccharide-induced Depressive-like Behaviors by Alleviating Neuroinflammation in the PFC via PI3K/Akt Signaling Pathway; Authors - Anhui Fu