Anticorps Polyclonal de lapin anti-TRPC1

• Phare
• Validé par KD/KO

TRPC1 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

10

N° de cat : 19482-1-AP

Synonymes

HTRP 1, Transient receptor protein 1, TRP 1, TRP1, TRPC1

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Galerie de données de validation

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  WB analysis of PC-3 using 19482-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 19482-1-AP (TRPC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 19482-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 19482-1-AP (TRPC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 19482-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 19482-1-AP (TRPC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 19482-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 19482-1-AP (TRPC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 19482-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 19482-1-AP (TRPC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 19482-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 19482-1-AP (TRPC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 19482-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 19482-1-AP (TRPC1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human brain using 19482-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 19482-1-AP (TRPC1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of PC-12 using 19482-1-AP

  Immunofluorescent analysis of PC-12 cells using 19482-1-AP (TRPC1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of PC-3 using 19482-1-AP

  1X10^6 PC-3 cells were stained with 0.2ug TRPC1 antibody (19482-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Applications testées

• Résultats positifs en WB: cellules PC-3, cellules SH-SY5Y, tissu cérébral humain, tissu hépatique de souris, tissu testiculaire humain
• Résultats positifs en IHC: tissu cérébral humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules PC-12
• Résultats positifs en cytométrie: cellules PC-3

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 10 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

19482-1-AP cible TRPC1 dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: transient receptor potential cation channel, subfamily C, member 1
• Masse moléculaire calculée: 91 kDa
• Poids moléculaire observé: 90 kDa, 130 kDa
• Numéro d’acquisition GenBank: NM_003304
• Symbole du gène: TRPC1
• Identification du gène (NCBI): 7220
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TRPC1, also named as TRP1, belongs to the transient receptor family and STrpC subfamily. TRPC1 is thought to form a receptor-activated non-selective calcium permeant cation channel. It has been reported that the mGluR1 evoked slow EPSC is mediated by the TRPC1 cation channel. It is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors.

Publications

Species	Application	Title
human	WB	Cell Res; Direct control of store-operated calcium channels by ultrafast laser.; Authors - Pan Cheng ; KD Validated
mouse	WB,IF	J Neuroinflammation; Upregulation of TRPC5 in hippocampal excitatory synapses improves memory impairment associated with neuroinflammation in microglia knockout IL-10 mice.; Authors - Shiji Huo
human	WB	J Biomed Sci; Chemoresistant ovarian cancer enhances its migration abilities by increasing store-operated Ca2+ entry-mediated turnover of focal adhesions.; Authors - Ho-Kai Huang
rat	WB	Sci Rep; Homer binds to Orai1 and TRPC channels in the neointima and regulates vascular smooth muscle cell migration and proliferation.; Authors - Shuping Jia
rat	WB	J Biochem Mol Toxicol; Galectin-3 mediates pulmonary vascular endothelial cell dynamics via TRPC1/4 under acute hypoxia.; Authors - Yumei Li
mouse	WB	Int J Mol Sci; STIM1 Knockout Enhances PDGF-Mediated Ca2+ Signaling through Upregulation of the PDGFR⁻PLCγ⁻STIM2 Cascade.; Authors - Tzu-Yu Huang

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