Anticorps Polyclonal de lapin anti-TWIST1

TWIST1 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications(19)

N° de cat : 18125-1-AP

Synonymes

ACS3, bHLHa38, BPES2, BPES3, H twist, SCS, TWIST, twist homolog 1 (Drosophila), Twist related protein 1, TWIST1

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Galerie de données de validation

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  WB analysis of PC-3 using 18125-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 18125-1-AP (TWIST1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of SGC-7901 using 18125-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 18125-1-AP (TWIST1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 18125-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 18125-1-AP (TWIST1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SGC-7901 using 18125-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 18125-1-AP (TWIST1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules PC-3, cellules MCF-7, cellules SGC-7901

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 17 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

18125-1-AP cible TWIST1 dans les applications de WB, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TWIST1 Protéine recombinante Ag12700
• Nom complet: twist homolog 1 (Drosophila)
• Masse moléculaire calculée: 202 aa, 21 kDa
• Poids moléculaire observé: 22 kDa, 28 kDa, 45-60 kDa
• Numéro d’acquisition GenBank: BC036704
• Symbole du gène: TWIST1
• Identification du gène (NCBI): 7291
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TWIST1, a basic helix-loop-helix protein which acts as a transcriptional regulator, plays a central role in differentiation of mesodermal during embryogenesis. It is also implicated in tumorigenesis, invasion and metastasis. TWIST1 forms both homodimers(45KD) and heterodimers with E2A E proteins, and dimer partner selection is a critical mediator of TWIST function. (PMID:14724576, 16502419).

Publications

Species	Application	Title
human,mouse	IHC,IF	Theranostics; Twist1 downregulation of PGC-1α decreases fatty acid oxidation in tubular epithelial cells, leading to kidney fibrosis.; Authors - Limin Liu
human	WB	Int J Biochem Cell Biol; Blue light emitting diodes irradiation causes cell death in colorectal cancer by inducing ROS production and DNA damage.; Authors - Gege Yan
human	WB	Cancer Lett; Downregulation of ACE2/Ang-(1-7)/Mas axis promotes breast cancer metastasis by enhancing store-operated calcium entry.; Authors - Changhui Yu
human	WB,IHC	Int J Cancer; Down-regulation of KIAA1199/CEMIP by miR-216a suppresses tumor invasion and metastasis in colorectal cancer.; Authors - Dejun Zhang
human	WB	Sci Rep; Correlations between TBL1XR1 and recurrence of colorectal cancer.; Authors - Hongda Liu
mouse	WB	Sci Rep; Conditional ablation of TGF-β signaling inhibits tumor progression and invasion in an induced mouse bladder cancer model.; Authors - Yu Liang

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