Anticorps Polyclonal de lapin anti-USP21

• Phare
• Validé par KD/KO

USP21 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, RIP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

10

N° de cat : 17856-1-AP

Synonymes

Deubiquitinating enzyme 21, Ubiquitin thiolesterase 21, USP16, USP21, USP23

---

Galerie de données de validation

• 

  WB analysis using 17856-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17856-1-AP (USP21 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17856-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17856-1-AP (USP21 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of recombinant ptotein using 17856-1-AP

  WB result of anti-UPS21 (17856-1-AP, 1:500) with HA-UPS21 and GST-UPS21 by Dr. Xicheng Mao.

• 

  WB analysis of RAW 264.7 using 17856-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 17856-1-AP (USP21 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 17856-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 17856-1-AP (USP21 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 17856-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17856-1-AP (USP21 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 17856-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17856-1-AP (USP21 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 17856-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 17856-1-AP (USP21 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 17856-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 17856-1-AP (USP21 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 17856-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 17856-1-AP (USP21 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 17856-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 17856-1-AP (USP21 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of RAW 264.7 using 17856-1-AP

  Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using 17856-1-AP (USP21 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Applications testées

• Résultats positifs en WB: cellules A549, cellules HeLa, cellules HepG2, cellules HuH-7, cellules RAW 264.7, ptotéine recombinante
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu cardiaque de souris, tissu pancréatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules RAW 264.7

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 6 publications below
• IHC: See 3 publications below
• CoIP: See 1 publications below
• RIP: See 1 publications below

Informations sur le produit

17856-1-AP cible USP21 dans les applications de WB, RIP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: USP21 Protéine recombinante Ag12316
• Nom complet: ubiquitin specific peptidase 21
• Masse moléculaire calculée: 565 aa, 63 kDa
• Poids moléculaire observé: 63 kDa
• Numéro d’acquisition GenBank: BC090946
• Symbole du gène: USP21
• Identification du gène (NCBI): 27005
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

USP21(Ubiquitin carboxyl-terminal hydrolase 21) is also named as USP23.The USPs belong to a subclass of the protein-deubiquitinating enzyme (DUB) superfamily that are categorized into five subclasses based on their ubiquitin-protease domains in the human genome and have been shown to be involved in a broad range of biological activities(PMID:19910467). USP21 is expressed at different levels in the tissues examined. The highest level of USP21 expression is found in the heart, pancreas, and skeletal muscle. USP21 messages could also be detected in the brain, placenta, liver, and kidney but are very low in the Lung through the northern blot(PMID:10799498).

Publications

Species	Application	Title
human	WB	Sci Transl Med; Guanosine diphosphate-mannose suppresses homologous recombination repair and potentiates antitumor immunity in triple-negative breast cancer; Authors - Jia-Han Ding
mouse	WB	EMBO J; Oligomerization-driven MLKL ubiquitylation antagonizes necroptosis.; Authors - Zikou Liu
human	IHC	Int J Mol Sci; PR-619, a General Inhibitor of Deubiquitylating Enzymes, Diminishes Cisplatin Resistance in Urothelial Carcinoma Cells through the Suppression of c-Myc: An In Vitro and In Vivo Study.; Authors - Fu-Shun Hsu
human	WB	Cell Death Dis; The deubiquitinase USP21 stabilizes MEK2 to promote tumor growth.; Authors - Wenjuan Li ; KD Validated
human	WB	J Virol; Deubiquitinating Enzyme USP21 Inhibits HIV-1 Replication by Downregulating Tat Expression.; Authors - Wenying Gao ; KD Validated
human	WB,CoIP	Pathol Int; Salt-like transcription factor 4 promotes laryngeal cancer progression through transcriptional activation of ubiquitin-specific protease 21 to stabilize Yin Yang 1; Authors - Qing-Dong Wang ; KD Validated