Anticorps Monoclonal anti-V5-tag

V5-tag Monoclonal Antibody for ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Protéine recombinante et plus (1)

Applications

WB, RIP, IP, CoIP, ELISA

Conjugaison

Non conjugué

CloneNo.

2B7A9

N° de cat : 66007-1-Ig

Synonymes



Applications testées

Dilution recommandée

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

66007-1-Ig cible V5-tag dans les applications de WB, RIP, IP, CoIP, ELISA et montre une réactivité avec des échantillons Protéine recombinante

Réactivité Protéine recombinante
Réactivité citéeHumain
Hôte / Isotype Mouse / IgG1
Clonalité Monoclonal
Type Anticorps
Immunogène Peptide
Nom complet V5-tag
Masse moléculaire calculée 1 kDa
Symbole du gène
Identification du gène (NCBI)
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par protéine G
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The antibody is developed against a synthetic 14 aa peptide (GKPIPNPLLGLDST), the V5 tag, which presents in many vectors.

Publications

SpeciesApplicationTitle
WB

Science

Structural insight into the SAM-mediated assembly of the mitochondrial TOM core complex.

Authors - Qiang Wang
WB

Immunity

Cytoplasmic DNA sensing by KU complex in aged CD4+ T cell potentiates T cell activation and aging-related autoimmune inflammation.

Authors - Yan Wang
humanWB

Nat Commun

K235 acetylation couples with PSPC1 to regulate the m6A demethylation activity of ALKBH5 and tumorigenesis

Authors - Xiao-Lan Zhang
WB

Nat Commun

The tight junction protein TJP1 regulates the feeding-modulated hepatic circadian clock.

Authors - Yi Liu

Dev Cell

Low-Level Saturated Fatty Acid Palmitate Benefits Liver Cells by Boosting Mitochondrial Metabolism via CDK1-SIRT3-CPT2 Cascade.

Authors - Lin Liu

Endocrinology

PMID: 30059978

Authors - Jessica Lee

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Tongbin (Verified Customer) (08-25-2020)

This V5 antibody detected my V5-tagged protein expressed in HEK293T cells. The band was very specific and strong.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK293T
FH

Zehua (Verified Customer) (02-27-2019)

Works well for IB and IP.

  • Applications: Western Blot, Immunoprecipitation,
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: 293T cell
V5-tag Antibody Western Blot,Immunoprecipitation, validation (1:2000 dilution) in 293T cell (Cat no:66007-1-Ig)