Anticorps Monoclonal anti-V5-tag

V5-tag Monoclonal Antibody for ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Protéine recombinante et plus (1)

Applications

WB, IP, CoIP, RIP, ELISA

Conjugaison

Non conjugué

Publications(38)

CloneNo.

2B7A9

Avis (2)

N° de cat : 66007-1-Ig

Synonymes

V5-tag

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Galerie de données de validation

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Applications publiées

• WB: See 19 publications below
• IP: See 4 publications below
• CoIP: See 3 publications below
• RIP: See 1 publications below

Informations sur le produit

66007-1-Ig cible V5-tag dans les applications de WB, IP, CoIP, RIP, ELISA et montre une réactivité avec des échantillons Protéine recombinante

• Réactivité: Protéine recombinante
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: V5 Tag
• Masse moléculaire calculée: 1 kDa
• Symbole du gène: V5 Tag
• Identification du gène (NCBI): 4
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The antibody is developed against a synthetic 14 aa peptide (GKPIPNPLLGLDST), the V5 tag, which presents in many vectors.

Publications

Species	Application	Title
	WB	Science; Structural insight into the SAM-mediated assembly of the mitochondrial TOM core complex.; Authors - Qiang Wang
	WB	Immunity; Cytoplasmic DNA sensing by KU complex in aged CD4+ T cell potentiates T cell activation and aging-related autoimmune inflammation.; Authors - Yan Wang
	WB	Nat Commun; K235 acetylation couples with PSPC1 to regulate the m6A demethylation activity of ALKBH5 and tumorigenesis; Authors - Xiao-Lan Zhang
	WB	Nat Commun; The tight junction protein TJP1 regulates the feeding-modulated hepatic circadian clock.; Authors - Yi Liu
		Dev Cell; Low-Level Saturated Fatty Acid Palmitate Benefits Liver Cells by Boosting Mitochondrial Metabolism via CDK1-SIRT3-CPT2 Cascade.; Authors - Lin Liu
		Endocrinology; PMID: 30059978; Authors - Jessica Lee

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tongbin (Verified Customer) (08-25-2020)

This V5 antibody detected my V5-tagged protein expressed in HEK293T cells. The band was very specific and strong.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T

FH

Zehua (Verified Customer) (02-27-2019)

Works well for IB and IP.

• Applications: Western Blot, Immunoprecipitation,
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: 293T cell

V5-tag Antibody Western Blot,Immunoprecipitation, validation (1:2000 dilution) in 293T cell (Cat no:66007-1-Ig)

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