Spectral flow cytometry is a next-generation approach that captures the full emission spectrum of each fluorochrome, enabling precise discrimination of highly overlapping signals and allowing for high-plex panel design.

In this webinar, Dr. Sylvia Okafor connected these principles to real-world application through a step-by-step walkthrough of a high-viability murine whole brain dissociation optimized for spectral cytometry.

Dr. Okafor covered:

• Dissection of the whole brain and gentle tissue processing

• Effective myelin removal

• Best practices for surface staining and panel design

• Guidance on spectral data acquisition and analysis