Tested Applications
| Positive WB detected in | human placenta tissue, mouse placenta tissue, A549 cells |
| Positive IP detected in | human placenta tissue |
| Positive IHC detected in | human intrahepatic cholangiocarcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10120-1-AP targets Calpain 6 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0160 Product name: Recombinant human CAPN6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 67-360 aa of BC000730 Sequence: NISNHQLTQGRLGHKPMVSAFSCLAVQESHWTKTIPNHKEQEWDPQKTEKYAGIFHFRFWHFGEWTEVVIDDLLPTINGDLVFSFSTSMNEFWNALLEKAYAKLLGCYEALDGLTITDIIVDFTGTLAETVDMQKGRYTELVEEKYKLFGELYKTFTKGGLICCSIESPNQEEQEVETDWGLLKGHTYTMTDIRKIRLGERLVEVFSAEKVYMVRLRNPLGRQEWSGPWSEISEEWQQLTASDRKNLGLVMSDDGEFWMSLEDFCRNFHKLNVCRNVNNPIFGRKELESVLGCW Predict reactive species |
| Full Name | calpain 6 |
| Calculated Molecular Weight | 75 kDa |
| Observed Molecular Weight | 66-75 kDa |
| GenBank Accession Number | BC000730 |
| Gene Symbol | Calpain 6 |
| Gene ID (NCBI) | 827 |
| RRID | AB_2290829 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y6Q1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Calpain 6 antibody 10120-1-AP | Download protocol |
| IP protocol for Calpain 6 antibody 10120-1-AP | Download protocol |
| WB protocol for Calpain 6 antibody 10120-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Calpain 6 Polyclonal antibody (Cat# 10120-1-AP) has 4 citations published in Cell Bioscience, Journal of Neuropathology & Experimental Neurology, Amino Acids, and American Journal of Reproductive Immunology. Research fields span embryonic development (temporomandibular joint), neurology (oligodendrocyte development under hypoxia-ischemia), nephrology (glycation in chronic kidney disease), and reproductive medicine (placental calpain in fetal growth restriction). Applications include WB, IF, and IHC in human and rat samples.
| Species | Application | Title |
|---|---|---|
Cell Biosci Single-cell RNA sequencing analysis of the temporomandibular joint condyle in 3 and 4-month-old human embryos | ||
J Neuropathol Exp Neurol MicroRNA Alteration in Developing Rat Oligodendrocyte Precursor Cells Induced by Hypoxia-Ischemia. | ||
Amino Acids Advanced glycation end products promote the progression of chronic kidney diseases by targeting calpain 6 | ||
Am J Reprod Immunol The investigation of calpain in human placenta with fetal growth restriction. |
