Tested Applications
| Positive WB detected in | C2C12 cells, mouse brain tissue, mouse heart tissue, rat heart tissue, mouse skeletal muscle, rat brain |
| Positive IHC detected in | mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse heart tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10145-1-AP targets TMOD1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0192 Product name: Recombinant human TMOD1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 105-359 aa of BC002660 Sequence: PVLESVTLEPELEEALANASDAELCDIAAILGMHTLMSNQQYYQALSSSSIMNKEGLNSVIKPTQYKPVPDEEPNSTDVEETLERIKNNDPKLEEVNLNNIRNIPIPTLKAYAEALKENSYVKKFSIVGTRSNDPVAYALAEMLKENKVLKTLNVESNFISGAGILRLVEALPYNTSLVEMKIDNQSQPLGNKVEMEIVSMLEKNATLLKFGYHFTQQGPRLRASNAMMNNNDLVRKRRLADLTGPIIPKCRSGV Predict reactive species |
| Full Name | tropomodulin 1 |
| Calculated Molecular Weight | 41 kDa |
| Observed Molecular Weight | 41 kDa |
| GenBank Accession Number | BC002660 |
| Gene Symbol | TMOD1 |
| Gene ID (NCBI) | 7111 |
| RRID | AB_2205316 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P28289 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Actin filaments control cell morphology. The length of these filaments is regulated in muscle and nonmuscle cell types by tropomodulins 1-4 (Tmod1-4), a family of proteins that cap the pointed ends of actin filaments. The molecular weight of Tmod 1 is about 40 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for TMOD1 antibody 10145-1-AP | Download protocol |
| IHC protocol for TMOD1 antibody 10145-1-AP | Download protocol |
| WB protocol for TMOD1 antibody 10145-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The TMOD1 Polyclonal antibody (Cat# 10145-1-AP) has 6 total citations. These appear in Communications Biology, Glia, American Journal of Surgical Pathology, Journal of Biological Chemistry, and PLoS One (×2). Research fields span childhood cardiomyopathy, oligodendroglial differentiation and myelination, hepatocellular adenoma versus carcinoma differentiation, postnatal cardiac development, and actin cytoskeleton organization in the heart.
| Species | Application | Title |
|---|---|---|
Glia S1P1 deletion in oligodendroglial lineage cells: Effect on differentiation and myelination. | ||
Am J Surg Pathol Identification of a unique gene expression signature that differentiates hepatocellular adenoma from well-differentiated hepatocellular carcinoma. | ||
J Biol Chem The actin-organizing formin protein Fhod3 is required for postnatal development and functional maintenance of the adult heart in mice. | ||
PLoS One Transgenic Expression of the Formin Protein Fhod3 Selectively in the Embryonic Heart: Role of Actin-Binding Activity of Fhod3 and Its Sarcomeric Localization during Myofibrillogenesis. | ||
PLoS One Expression and subcellular localization of mammalian formin Fhod3 in the embryonic and adult heart. |
