Tested Applications
| Positive WB detected in | HeLa cells, Jurkat cells, mouse brain tissue, rat brain tissue |
| Positive IHC detected in | human gliomas tissue, rat kidney tissue, rat testis tissue, mouse kidney tissue, human gliomas tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10247-2-AP targets GNB1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0313 Product name: Recombinant human GNB1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 2-339 aa of BC004186 Sequence: SELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIW Predict reactive species |
| Full Name | guanine nucleotide binding protein (G protein), beta polypeptide 1 |
| Calculated Molecular Weight | 37 kDa |
| Observed Molecular Weight | 37 kDa |
| GenBank Accession Number | BC004186 |
| Gene Symbol | GNB1 |
| Gene ID (NCBI) | 2782 |
| RRID | AB_2111820 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P62873 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
GNB1(Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1) belongs to the WD repeat G protein beta family.The guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems and the beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.GNB1 can form dimers with all gamma subunits analyzed(PMID:12782285).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for GNB1 antibody 10247-2-AP | Download protocol |
| IHC protocol for GNB1 antibody 10247-2-AP | Download protocol |
| WB protocol for GNB1 antibody 10247-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The GNB1 antibody (Cat# 10247-2-AP) has 13 citations spanning journals including Cell, PLoS Biology, PLoS Pathogens, Phytomedicine, J Bone Miner Res, Cancer Sci, Toxicology, iScience, Neuropharmacology, Exp Eye Res, Epilepsy Res, Bioengineered, and Dev Dyn. Research fields cover membrane dynamics, pancreatitis, morphine dependence, osteogenesis, lung and liver carcinogenesis, avian influenza, GABAergic signaling, malaria, photoreceptor development, epilepsy, colon cancer, and neural tube defects.
| Species | Application | Title |
|---|---|---|
Phytomedicine Kaempferol functionally reprograms CD47 signaling to promote cytoprotection and attenuate oxeiptosis in severe acute pancreatitis. | ||
PLoS Biol KCTD proteins regulate morphine dependence via heterologous sensitization of adenylyl cyclase 1 in mice | ||
J Bone Miner Res G protein inhibitory alpha subunits 1 and 3 regulate Wnt/beta-catenin signaling to promote osteogenesis and bone formation. | ||
Toxicology KCNJ15 inhibits chemical-induced lung carcinogenesis and progression through GNB1 mediated Hippo pathway | ||
PLoS Pathog H9N2 virus-derived M1 protein promotes H5N6 virus release in mammalian cells: Mechanism of avian influenza virus inter-species infection in humans. |
Reviews
What customers say
The single review for this product is a 5-star rating from a Western Blot application. The reviewer expressed strong satisfaction, stating they would give it "three thumbs up" if possible, indicating a highly positive experience with the product's performance.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Nick (Verified Customer) (04-20-2022) | If I could give this three thumbs up, I would.
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