Tested Applications
| Positive WB detected in | BxPC-3 cells, SW 1990 cells, HeLa cells |
| Positive IHC detected in | human liver tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10251-1-AP targets TIP30 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0353 Product name: Recombinant human TIP30 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 82-276 aa of BC002439 Sequence: TLIGRRKLTFDEEAYKNVNQEVVDFEKLDDYASAFQGHDVGFCCLGTTRGKAGAEGFVRVDRDYVLKSAELAKAGGCKHFNLLSSKGADKSSNFLYLQVKGEVEAKVEELKFDRYSVFRPGVLLCDRQESRPGEWLVRKFFGSLPDSWARGHSVPVVTVVRAMLNNVVRPRDKQMELLENKAIHDLGKAHGSLKP Predict reactive species |
| Full Name | HIV-1 Tat interactive protein 2, 30kDa |
| Calculated Molecular Weight | 27 kDa |
| Observed Molecular Weight | 30 kDa |
| GenBank Accession Number | BC002439 |
| Gene Symbol | TIP30 |
| Gene ID (NCBI) | 10553 |
| RRID | AB_2279817 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BUP3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TIP30/CC3 was first identified and characterized as a "candidate" tumor-suppressor gene in 1997. TIP30 is involved in the control of cell apoptosis, growth, metastasis, angiogenesis, DNA repair, and tumor cell metabolism.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for TIP30 antibody 10251-1-AP | Download protocol |
| IHC protocol for TIP30 antibody 10251-1-AP | Download protocol |
| WB protocol for TIP30 antibody 10251-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The TIP30 Polyclonal antibody (Cat# 10251-1-AP) has 2 citations. These appear in Nature Communications (2025) and Frontiers in Cell and Developmental Biology (2021). The research fields span lipid metabolism and remyelination (foam cell formation via ABCA1 degradation) and reproductive biology (trophoblast necroptosis and preeclampsia pathogenesis). Both studies utilized the antibody for Western blot applications, with one in human samples and the other in mouse.
| Species | Application | Title |
|---|---|---|
Nat Commun UBE3A promotes foam cell formation and counters remyelination by targeting ABCA1 for proteasomal degradation | ||
Front Cell Dev Biol Role of GRK2 in Trophoblast Necroptosis and Spiral Artery Remodeling: Implications for Preeclampsia Pathogenesis. |
