Tested Applications
| Positive WB detected in | mouse kidney tissue, human heart tissue, human stomach tissue, mouse stomach tissue, mouse heart tissue, HEK-293 cells, MCF-7 cells, human placenta tissue |
| Positive IP detected in | mouse heart tissue |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10393-1-AP targets COMMD5 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0592 Product name: Recombinant human COMMD5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 4-159 aa of BC003055 Sequence: VGAATPYLHHPGDSHSGRVSFLGAQLPPEVAAMARLLGDLDRSTFRKLLKFVVSSLQGEDCREAVQRLGVSANLPEEQLGALLAGMHTLLQQALRLPPTSLKPDTFRDQLQELCIPQDLVGDLASVVFGSQRPLLDSVAQQQGAWLPHVADFRWRV Predict reactive species |
| Full Name | COMM domain containing 5 |
| Calculated Molecular Weight | 25 kDa |
| Observed Molecular Weight | 25 kDa |
| GenBank Accession Number | BC003055 |
| Gene Symbol | COMMD5 |
| Gene ID (NCBI) | 28991 |
| RRID | AB_2083555 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9GZQ3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
COMM domain containing 5 (COMMD5, synonyms: HCARG, HT002) is a hypertension-related calcium-regulated gene. COMMD5 is negatively regulated by extracellular calcium concentration, and its basal mRNA levels were higher in hypertensive animals. Tissue distribution of COMMD5 shows a preponderance in the heart, stomach, jejunum, kidney (tubular fraction), liver, and adrenal gland (mainly in the medulla). COMMD5 mRNA is significantly more expressed in adult than in fetal organs, and its levels are decreased in tumors and cancerous cell lines. COMMD5 protein has no transmembrane domain, but contains 67% -helix content, a calcium-binding site, four putative "leucine zipper" motifs, and a nuclear receptor-binding domain. At the subcellular level, COMMD5 shows a nuclear localization.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for COMMD5 antibody 10393-1-AP | Download protocol |
| IP protocol for COMMD5 antibody 10393-1-AP | Download protocol |
| WB protocol for COMMD5 antibody 10393-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-COMMD5 antibody (Cat# 10393-1-AP) has 12 total citations. Publications appear in journals including J Extracell Vesicles, Sci Immunol, J Am Soc Nephrol, Cell Reports, Mol Metab, Sci Rep, Oncol Rep, Am J Physiol Renal Physiol, Anticancer Res, Oncotarget, and eLife. Research fields span extracellular vesicle uptake, autoimmunity, renal injury and physiology, endosomal trafficking, insulin secretion, inflammation, gastric cancer, nephrotoxicity, renal cell carcinoma, and lipid-mediated recycling pathways.
| Species | Application | Title |
|---|---|---|
J Extracell Vesicles Genome-Wide CRISPR/Cas9 Screening Identifies the COMMANDER Recycling Complex as a Key Player in EV Uptake.
| ||
Sci Immunol Celastrol suppresses humoral immune responses and autoimmunity by targeting the COMMD3/8 complex | ||
Cell Rep COMMD5/HCaRG Hooks Endosomes on Cytoskeleton and Coordinates EGFR Trafficking.
| ||
Mol Metab Genome-wide CRISPR screen identified a role for commander complex mediated ITGB1 recycling in basal insulin secretion. | ||
Sci Rep Inhibition of lipopolysaccharide-induced inflammation by trophoblast-conditioned medium and trophoblast-derived extracellular vesicles in human middle ear epithelial cells |
