Tested Applications
| Positive WB detected in | HeLa cells, A431 cells, mouse colon tissue, rat colon tissue |
| Positive IP detected in | A431 cells |
| Positive IHC detected in | human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10565-1-AP targets HIC5 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0868 Product name: Recombinant human TGFB1I1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-230 aa of BC001830 Sequence: MPRSGAPKERPAEPLTPPPSYGHQPQTGSGESSGASGDKDHLYSTVCKPRSPKPAAPAAPPFSSSSGVLGTGLCELDRLLQELNATQFNITDEIMSQFPSSKVASGEQKEDQSEDKKRPSLPSSPSPGLPKASATSATLELDRLMASLSDFRVQNHLPASGPTQPPVVSSTNEGSPSPPEPTGKGSLDTMLGLLQSDLSRRGVPTQAKGLCGSCNKPIAGQVVTALGRAW Predict reactive species |
| Full Name | transforming growth factor beta 1 induced transcript 1 |
| Calculated Molecular Weight | 48 kDa |
| Observed Molecular Weight | 48-50 kDa |
| GenBank Accession Number | BC001830 |
| Gene Symbol | HIC5 |
| Gene ID (NCBI) | 7041 |
| RRID | AB_2202045 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O43294 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for HIC5 antibody 10565-1-AP | Download protocol |
| IHC protocol for HIC5 antibody 10565-1-AP | Download protocol |
| IP protocol for HIC5 antibody 10565-1-AP | Download protocol |
| WB protocol for HIC5 antibody 10565-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-HIC-5 antibody (Cat# 10565-1-AP) has accumulated 14 citations published in journals such as Cell Metabolism, Theranostics, Cell Death & Disease, Journal of Cell Biology, EMBO Reports, Acta Pharmacologica Sinica, Aging, European Journal of Pharmacology, and others. Associated research fields include cardiac metabolism, vascular biology, renal fibrosis, multiple cancer types (esophageal, breast, osteosarcoma, bladder, colorectal, hepatocellular), cell adhesion, liquid-liquid phase separation, and mechanotransduction.
| Species | Application | Title |
|---|---|---|
Cell Metab O-GlcNAcylation-mediated endothelial metabolic memory contributes to cardiac damage via small extracellular vesicles | ||
Theranostics O-GlcNAcylation-dependent liquid-liquid phase separation regulates the nuclear translocation of YAP to exacerbate vascular neointimal hyperplasia | ||
Cell Death Dis HIC-5 in cancer-associated fibroblasts contributes to esophageal squamous cell carcinoma progression. | ||
Acta Pharmacol Sin GPR97 deficiency ameliorates renal interstitial fibrosis in mouse hypertensive nephropathy | ||
J Cell Biol Paxillin phase separation promotes focal adhesion assembly and integrin signaling | ||
EMBO Rep An ULK1/2-PXN mechanotransduction pathway suppresses breast cancer cell migration |
