ITPK1 Polyclonal antibody

ITPK1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 10568-1-AP

Publications(1)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

Ins(1,3,4)P(3) 5/6 kinase, ITPK1, ITRPK1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

-/ -

Freight/Packing: -

Quantity

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Filter: AllWBIHC

WB analysis of human brain using 10568-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 10568-1-AP (ITPK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of mouse eye using 10568-1-AP
mouse eye tissue were subjected to SDS PAGE followed by western blot with 10568-1-AP (ITPK1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HEK-293 using 10568-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 10568-1-AP (ITPK1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human brain using 10568-1-AP
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10568-1-AP (ITPK1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human brain using 10568-1-AP
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10568-1-AP (ITPK1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Publications (1)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	human brain tissue, HEK-293 cells, mouse eye tissue
Positive IHC detected in	human brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10568-1-AP targets ITPK1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag0834 Product name: Recombinant human ITPK1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-314 aa of BC007428 Sequence: MQTFLKGKRVGYWLSEKKIKKLNFQAFAELCRKRGMEVVQLNLSRPIEEQGPLDVIIHKLTDVILEADQNDSQSLELVHRFQEYIDAHPETIVLDPLPAIRTLLDRSKSYELIRKIEAYMEDDRICSPPFMELTSLCGDDTMRLLEKNGLTFPFICKTRVAHGTNSHEMAIVFNQEGLNAIQPPCVVQNFINHNAVLYKVFVVGESYTVVQRPSLKNFSAGTSDRESIFFNSHNVSKPESSSVLTELDKIEGVFERPSDEVIRELSRALRQALGVSLFGIDIIINNQTGQHAVIDINAFPGDCQVCFIEGWKTD Predict reactive species
Full Name	inositol 1,3,4-triphosphate 5/6 kinase
Calculated Molecular Weight	48 kDa
Observed Molecular Weight	45 kDa
GenBank Accession Number	BC007428
Gene Symbol	ITPK1
Gene ID (NCBI)	3705
RRID	AB_2129832
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q13572
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for ITPK1 antibody 10568-1-AP	Download protocol
WB protocol for ITPK1 antibody 10568-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

Species	Application	Title
mouse	IHC	PeerJ Identification of diabetes related phenotype and diagnostic biomarkers in coronary artery disease. Authors - Gulandanmu Aihemaiti View Article