PLCD4 Polyclonal antibody

PLCD4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10589-2-AP

Synonyms

hPLCD4, Phospholipase C delta 4, phospholipase C, delta 4, PLC delta 4, PLCD4



Tested Applications

Positive WB detected inmouse skeletal muscle tissue, rat skeletal muscle tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10589-2-AP targets PLCD4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PLCD4 fusion protein Ag0937
Full Name phospholipase C, delta 4
Calculated Molecular Weight 85-90 kDa
Observed Molecular Weight100-110 kDa
GenBank Accession NumberBC006355
Gene Symbol PLCD4
Gene ID (NCBI) 84812
RRIDAB_2163398
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PLCD4 is a member of the delta class of phospholipase C enzymes. Phospholipase C enzymes play a critical role in many cellular processes by hydrolyzing phosphatidylinositol 4,5-bisphosphate into two intracellular second messengers, inositol 1,4,5-trisphosphate and diacylglycerol. Expression of this gene may be a marker for cancer.

Protocols

Product Specific Protocols
WB protocol for PLCD4 antibody 10589-2-APDownload protocol
IHC protocol for PLCD4 antibody 10589-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols