Tested Applications
| Positive WB detected in | MOLT-4 cells, HEK-293 cells, Jurkat cells, K-562 cells, Caco-2 cells |
| Positive IHC detected in | mouse colon tissue, rat ovary tissue, human placenta tissue, rat colon tissue, mouse ovary tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10683-1-AP targets CDC23 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1068 Product name: Recombinant human CDC23 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-126 aa of BC005258 Sequence: MVPVAVTAAVAPVLSINSDFSDLREIKKQLLLIAGLTRERGLLHSSKWSAELAFSLPALPLAELQPPPPITEEDAQDMDAYTLAKAYFDVKEYDRAAHFLHGCNSKKAYFLYMYSRYLVRAILKCH Predict reactive species |
| Full Name | cell division cycle 23 homolog (S. cerevisiae) |
| Calculated Molecular Weight | 68 kDa |
| Observed Molecular Weight | 69 kDa |
| GenBank Accession Number | BC005258 |
| Gene Symbol | CDC23 |
| Gene ID (NCBI) | 8697 |
| RRID | AB_2075008 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UJX2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CDC23(cell division cycle protein 23 homolog) is also named ANAPC8 and belongs to the APC8/CDC23 family. It is a subunit of the anaphase-promoting complex (APC), which functions at the metaphase-to-anaphase transition of the cell cycle and is regulated by spindle checkpoint proteins. It can be phosphorylated and the phosphorylation of Thr-562 occurs specifically during mitosis(PMID:19690332). It has 3 isoforms produced by alternative splicing with the molecular weight of 69 kDa, 17 kDa, and 56 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for CDC23 antibody 10683-1-AP | Download protocol |
| WB protocol for CDC23 antibody 10683-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CDC23 Polyclonal antibody (Cat# 10683-1-AP) has a total of 2 citations. These appear in Advanced Science and PLoS Pathogens. The cited research covers cell cycle regulation and mitotic checkpoint mechanisms, as well as viral biology involving murine cytomegalovirus cell cycle regulators. Applications used in these studies include Western blotting and immunofluorescence.
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) CCDC68 Maintains Mitotic Checkpoint Activation by Promoting CDC20 Integration into the MCC | ||
PLoS Pathog A murine cytomegalovirus cell cycle regulator (m54.5p) evolved within the conserved viral DNA polymerase gene. |
