PSG4 Polyclonal antibody

PSG4 Polyclonal Antibody for IHC, IF-P, ELISA

Cat No. 10902-2-AP

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF-P, ELISA

Synonyms

CGM4, CGM4, PSG9, Pregnancy-specific beta-1-glycoprotein 4, Pregnancy-specific beta-1-glycoprotein 9, Pregnancy-specific glycoprotein 4

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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Filter: AllIHCIF-P

IHC staining of human placenta using 10902-2-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10902-2-AP (PSG4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human placenta using 10902-2-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10902-2-AP (PSG4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of human placenta using 10902-2-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using PSG4 antibody (10902-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of human placenta using 10902-2-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using PSG4 antibody (10902-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

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MSDS SDS

Tested Applications

Positive IHC detected in	human placenta tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	human placenta tissue

Recommended dilution

Application	Dilution
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10902-2-AP targets PSG4 in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag1167 Product name: Recombinant human PSG4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 120-419 aa of BC008405 Sequence: YTLHIIKGDDGTRGVTGRFTFTLHLETPKPSISSSNLNPRETMEAVSLTCDPETPDASYLWWMNGQSLPMTHSLKLSETNRTLFLLGVTKYTAGPYECEIRNPVSASRSDPVTLNLLPKLSKPYITINNLNPRENKDVLTFTCEPKSENYTYIWWLNGQSLPVSPRVKRPIENRILILPNVTRNETGPYQCEIRDRYGGIRSDPVTLNVLYGPDLPSIYPSFTYYRSGENLYLSCFAESNPRAQYSWTINGKFQLSGQKLSIPQITTKHSGLYACSVRNSATGKESSKSITVKVSDWILP Predict reactive species
Full Name	pregnancy specific beta-1-glycoprotein 4
Calculated Molecular Weight	47 kDa
GenBank Accession Number	BC008405
Gene Symbol	PSG4
Gene ID (NCBI)	5672
RRID	AB_2171057
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q00888
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IF protocol for PSG4 antibody 10902-2-AP	Download protocol
IHC protocol for PSG4 antibody 10902-2-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The PSG4 polyclonal antibody (Cat# 10902-2-AP) has 1 citation. It was cited in Developmental Cell (IF 9.2) in a 2022 study by Zunpeng Liu et al., titled "Large-scale chromatin reorganization reactivates placenta-specific genes that drive cellular aging." The research field encompasses developmental biology, chromatin dynamics, and cellular aging, with the antibody applied in Western blot and immunofluorescence in human samples.

Species	Application	Title
human	WB,IF	Dev Cell Large-scale chromatin reorganization reactivates placenta-specific genes that drive cellular aging. Authors - Zunpeng Liu View Article