Tested Applications
| Positive WB detected in | human saliva, HEK-293 cells, human pancreas tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | mouse brain tissue, human prostate hyperplasia tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10933-1-AP targets Lactotransferrin/LTF in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, bovine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1379 Product name: Recombinant human LTF protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 346-711 aa of BC015822 Sequence: TAIQNLRKSEEEVAARRARVVWCAVGEQELRKCNQWSGLSEGSVTCSSASTTEDCIALVLKGEADAMSLDGGYVYTAGKCGLVPVLAENYKSQQSSDPDPNCVDRPVEGYLTVAVVRRSDTSLTWNSVKGKKSCHTAVDRTAGWNIPMGLLFNQTGSCKFDEYFSQSCAPGSDPRSNLCALCIGDEQGENKCEPNSNERYYGYTGAFRCLAENAGDVAFVKDVTVLQNTDGNNNEAWAKDLKLADFALLCLDGKRKPVTEARSCHLAMAPNHAVVSRMDKVERLKQVLLHQQAKFGRNGSDCPDKFCLFQSETKNLLFNDNTECLARLHGKTTYEKYLGPQYVAGITNLKKCSTSPLLEACEFLRK Predict reactive species |
| Full Name | lactotransferrin |
| Calculated Molecular Weight | 80 kDa |
| Observed Molecular Weight | 30,51,80 kDa |
| GenBank Accession Number | BC015822 |
| Gene Symbol | LTF |
| Gene ID (NCBI) | 4057 |
| RRID | AB_2139227 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P02788 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
LTF(Lactotransferrin) is also named as GIG12, LF and belongs to the transferrin family. It is an iron binding transport protein which can bind two atoms of ferric iron in association with the binding of an anion, usually bicarbonate. LTF is also an important protein component of the innate immune system that is broadly distributed within the body fluids. It can contribute to the activation of both the innate and adaptive immune responses by promoting the recruitment of leukocytes and activation of dendritic cells(PMID:18453607). This protein has 2 isoforms produced by alternative promoter usage. The human LTF can be isolated two fragments, an N-terminal tryptic fragment having an MW of 30 kDa and a C-terminal tryptic fragment having an MW of 50 kDa by a mild tryptic digest(PMID:3790094).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Lactotransferrin/LTF antibody 10933-1-AP | Download protocol |
| IP protocol for Lactotransferrin/LTF antibody 10933-1-AP | Download protocol |
| WB protocol for Lactotransferrin/LTF antibody 10933-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Lactotransferrin/LTF Polyclonal antibody (Cat# 10933-1-AP) has 18 citations published in journals including Nature Genetics, Science Advances, Bioactive Materials, Biochemical Pharmacology, International Immunopharmacology, Cancer Science, Journal of Cellular and Molecular Medicine, Experimental Cell Research, and Clinical and Experimental Immunology. Research fields encompass oncology (thyroid, lung, gastric, breast, hepatocellular, glioblastoma), cystic fibrosis, acute kidney injury, ferroptosis, autophagy, cartilage degeneration, spinal neuron function, trauma response, and sustainable lactoferrin bioproduction.
| Species | Application | Title |
|---|---|---|
Bioact Mater Passively-targeted mitochondrial tungsten-based nanodots for efficient acute kidney injury treatment | ||
Sci Adv Mucus concentration-dependent biophysical abnormalities unify submucosal gland and superficial airway dysfunction in cystic fibrosis. | ||
Bioresour Technol Integrated bioprocess engineering of Chlamydomonas reinhardtii for sustainable production of human lactoferrin via direct Air-Captured CO(2) conversion. | ||
Cancer Cell Int Identification and validation of an immune-related prognostic signature and key gene in papillary thyroid carcinoma. | ||
Biochem Pharmacol PX-478 induces apoptosis in acute myeloid leukemia under hypoxia by inhibiting the PI3K/AKT/mTOR pathway through downregulation of GBE1 |
