Tested Applications
| Positive WB detected in | mouse heart tissue, mouse liver tissue, PC-3 cells |
| Positive IP detected in | PC-3 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11062-1-AP targets TIMM17B in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1504 Product name: Recombinant human TIMM17B protein Source: e coli.-derived, PKG Tag: GST Domain: 1-172 aa of BC010142 Sequence: MEEYAREPCPWRIVDDCGGAFTMGVIGGGVFQAIKGFRNAPVGIRHRLRGSANAVRIRAPQIGGSFAVWGGLFSTIDCGLVRLRGKEDPWNSITSGALTGAVLAARSGPLAMVGSAMMGGILLALIEGVGILLTRYTAQQFRNAPPFLEDPSQLPPKDGTPAPGYPSYQQYH Predict reactive species |
| Full Name | translocase of inner mitochondrial membrane 17 homolog B (yeast) |
| Calculated Molecular Weight | 18 kDa |
| Observed Molecular Weight | 17-20 kDa |
| GenBank Accession Number | BC010142 |
| Gene Symbol | TIMM17B |
| Gene ID (NCBI) | 10245 |
| RRID | AB_2201995 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O60830 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for TIMM17B antibody 11062-1-AP | Download protocol |
| IP protocol for TIMM17B antibody 11062-1-AP | Download protocol |
| WB protocol for TIMM17B antibody 11062-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The TIMM17B Polyclonal antibody (Cat# 11062-1-AP) has 10 citations published in journals including Cell Metabolism, Molecular Cell, Cell Reports, Journal of Cell Biology, Scientific Reports, American Journal of Physiology, Cancer Research Communications, Infection and Immunity, and Cellular and Molecular Biology. Research fields span mitochondrial protein import machinery, metabolic regulation, immune responses, Sengers syndrome, and cancer (lung and breast).
| Species | Application | Title |
|---|---|---|
Cell Metab Acylglycerol Kinase Maintains Metabolic State and Immune Responses of CD8+ T Cells. | ||
Mol Cell Acylglycerol Kinase Mutated in Sengers Syndrome Is a Subunit of the TIM22 Protein Translocase in Mitochondria. | ||
Mol Cell Sengers Syndrome-Associated Mitochondrial Acylglycerol Kinase Is a Subunit of the Human TIM22 Protein Import Complex. | ||
Cell Rep TIM23 facilitates PINK1 activation by safeguarding against OMA1-mediated degradation in damaged mitochondria | ||
J Cell Biol ROMO1 is a constituent of the human presequence translocase required for YME1L protease import. | ||
Sci Rep The presence of disulfide bonds reveals an evolutionarily conserved mechanism involved in mitochondrial protein translocase assembly. |
Reviews
What customers say
The single review for this product was posted by Angela Zhu on December 27, 2024. She used it for Western Blot at a 1:1000 dilution on heart tissue (lot 00018765) and rated it 4 out of 5 stars, commenting simply "good." Overall, the limited feedback suggests the antibody performs adequately for WB applications, though the sparse review base makes it difficult to draw broader conclusions about consistency or performance across other applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Angela (Verified Customer) (12-27-2024) | good
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