Tested Applications
| Positive WB detected in | Jurkat cells, K-562 cells |
| Positive IP detected in | K-562 cells |
| Positive IHC detected in | human lung cancer tissue, human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11088-1-AP targets TID1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1558 Product name: Recombinant human DNAJA3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 181-480 aa of BC011855 Sequence: DPEELFRKIFGEFSSSSFGDFQTVFDQPQEYFMELTFNQAAKGVNKEFTVNIMDTCERCNGKGNEPGTKVQHCHYCGGSGMETINTGPFVMRSTCRRCGGRGSIIISPCVVCRGAGQAKQKKRVMIPVPAGVEDGQTVRMPVGKREIFITFRVQKSPVFRRDGADIHSDLFISIAQALLGGTARAQGLYETINVTIPPGTQTDQKIRMGGKGIPRINSYGYGDHYIHIKIRVPKRLTSRQQSLILSYAEDETDVEGTVNGVTLTSSGGSTMDSSAGSKARREAGEDEEGFLSKLKKMFTS Predict reactive species |
| Full Name | DnaJ (Hsp40) homolog, subfamily A, member 3 |
| Calculated Molecular Weight | 480 aa, 53 kDa |
| Observed Molecular Weight | 40 kDa |
| GenBank Accession Number | BC011855 |
| Gene Symbol | TID1 |
| Gene ID (NCBI) | 9093 |
| RRID | AB_2293102 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96EY1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for TID1 antibody 11088-1-AP | Download protocol |
| IP protocol for TID1 antibody 11088-1-AP | Download protocol |
| WB protocol for TID1 antibody 11088-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The TID1 polyclonal antibody (Cat# 11088-1-AP) has 3 citations published in Nature Communications, Cell Molecular Gastroenterology and Hepatology, and iScience. The cited research spans proteogenomic characterization of skull-base chordoma, gut microbiota-derived propionate's role in hepatic N2 neutrophils in Wilson's disease, and human HSPE1's role in OPA1 processing independent of HSPD1. All citations used the antibody for Western blot in human samples.
| Species | Application | Title |
|---|---|---|
Cell Mol Gastroenterol Hepatol Gut Microbiota-Derived Propionate Governs Hepatic N2 Neutrophils in Wilson's Disease. | ||
