Tested Applications
| Positive WB detected in | SGC-7901 cells, Jurkat cells |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11151-1-AP targets MYEOV in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1498 Product name: Recombinant human MYEOV protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-313 aa of BC011815 Sequence: MALRICVTYTPALPIGLCTRCCLCLEQSPSWCHCLRGVSFLTFHLHQSVPLGDRDSLLMFTRQAGHFVEGSKAGRSRGRLCLSQALRVAVRGAFVSLWFAAGAGDRERNKGDKGAQTGAGLSQEAEDVDVSRARRVTDAPQGTLCGTGNRNSGSQSARAVGVAHLGEAFRVGVEQAISSCPEEVHGRHGLSMEIMWARMDVALRSPGRGLLAGAGALCVTLAESSCPDYERGRRACLTLHRHPTPHCSTWGLPLRVAGSWLTVVTVEALGGWRMGVRRTGQVGPTMHPPPVSGASPLLLHHLLLLLLIIILTC Predict reactive species |
| Full Name | myeloma overexpressed (in a subset of t(11;14) positive multiple myelomas) |
| Calculated Molecular Weight | 33 kDa |
| Observed Molecular Weight | 55-60 kDa |
| GenBank Accession Number | BC011815 |
| Gene Symbol | MYEOV |
| Gene ID (NCBI) | 26579 |
| RRID | AB_671520 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96EZ4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MYEOV, also named as OCIM, was originally isolated by the application of the NIH/3T3 tumorigenicity assay with DNA from a gastric carcinoma. It is a prognostic factor for patients with multiple myeloma, in part through a role of MYEOV in the control of MMC proliferation. MYEOV is a candidate oncogene activated in the amplification core located proximal toCCND1. The observed MW of this protein is 55 kDa(PMID: 20854874 ).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for MYEOV antibody 11151-1-AP | Download protocol |
| WB protocol for MYEOV antibody 11151-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MYEOV Polyclonal antibody (Cat# 11151-1-AP) has 4 citations appearing in Cell Oncology, Cancers (Basel), Cell Cycle, and Experimental Hematology. The associated research fields include pancreatic cancer prognostic biomarker identification, MYEOV gene network analysis in pancreatic cancer, MYEOV overexpression in pancreatic ductal adenocarcinoma, and MYEOV as a prognostic factor in multiple myeloma.
| Species | Application | Title |
|---|---|---|
Cell Oncol (Dordr) Genomic analysis and filtration of novel prognostic biomarkers based on metabolic and immune subtypes in pancreatic cancer | ||
Cancers (Basel) Identification of MYEOV-Associated Gene Network as a Potential Therapeutic Target in Pancreatic Cancer | ||
Cell Cycle Overexpression of MYEOV predicting poor prognosis in patients with pancreatic ductal adenocarcinoma. | ||
