Tested Applications
| Positive WB detected in | A431 cells, HEK-293 cells, A549 cells, HeLa cells, HepG2 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:10-1:100 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11281-1-AP targets EIF4G3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1811 Product name: Recombinant human EIF4G3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 197-515 aa of BC030578 Sequence: SQVPEHSPVVYGTVESAHLAASTPVTAASDQKQEEKPKPDPVLKSPSPVLRLVLSGEKKEQEGQTSETTAIVSIAELPLPPSPTTVSSVARSTIAAPTSSALSSQPIFTTAIDDRCELSSPREDTIPIPSLTSCTETSDPLPTNENDGDICKKPCSVAPNDIPLVSSTNLINEINGVSEKLSATESIVEIVKQEVLPLTLELEILENPPEEMKLECIPAPITPSTVPSFPPTPPTPPASPPHTPVIVPAAATTVSSPSAAITVQRVLEEDESIRTCLSEDAKEIQNKIEVEADGQTEEILDSQNLNSRRSPVPETSNEC Predict reactive species |
| Full Name | eukaryotic translation initiation factor 4 gamma, 3 |
| Calculated Molecular Weight | 177 kDa |
| Observed Molecular Weight | 70 kDa, 250 kDa |
| GenBank Accession Number | BC030578 |
| Gene Symbol | EIF4G3 |
| Gene ID (NCBI) | 8672 |
| RRID | AB_2877754 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O43432 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Eukaryotic translation initiation factor 4 gamma, 3 (EIF4G3) may be involved in the recognition of the mRNA cap. The protein migrated at approximately 70 kDa and/or 250 kDa in SDS-PAGE.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for EIF4G3 antibody 11281-1-AP | Download protocol |
| IHC protocol for EIF4G3 antibody 11281-1-AP | Download protocol |
| IP protocol for EIF4G3 antibody 11281-1-AP | Download protocol |
| WB protocol for EIF4G3 antibody 11281-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The EIF4G3 Polyclonal Antibody (Cat# 11281-1-AP) has 7 citations published in Cancer Research, Advanced Science, Science Advances, Molecular Biotechnology, Theriogenology, and Japanese Journal of Clinical Oncol. Research fields span oncology (renal cell carcinoma, lymphoma, melanoma), reproductive biology and meiosis, mRNA epigenetic regulation, translation control, and endogenous protein function characterization. Applications cited include WB, IF, and IHC across human, mouse, and pig species.
| Species | Application | Title |
|---|---|---|
Cancer Res Fatty Acid Oxidation Mediated by Malonyl-CoA Decarboxylase Represses Renal Cell Carcinoma Progression | ||
Adv Sci (Weinh) Primate-Specific DAZ Regulates Translation of Cell Proliferation-Related mRNAs and is Essential for Maintenance of Spermatogonia | ||
Adv Sci (Weinh) m6A Reader hnRNPA2B1 Modulates Late Pachytene Progression in Male Meiosis Through Post-Transcriptional Control | ||
Sci Adv High-throughput tagging of endogenous loci for rapid characterization of protein function | ||
Mol Biotechnol Expression Profile and Prognostic Significance of Pivotal Regulators for N7-Methylguanosine Methylation in Diffuse Large B-Cell Lymphoma | ||
Theriogenology Promoter-associated lncRNA pancEIF4G3 regulates embryonic genome activation in porcine early embryos. |
